A microfabrication-based dynamic array cytometer
A microfabrication-based dynamic array cytometer
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DOI：
10.1021/ac0256235
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发表时间：
2002-08-15
影响因子：
7.4
通讯作者：
Schmidt, MA
中科院分区：
文献类型：
--
作者：
Voldman, J;Gray, ML;Schmidt, MA
我们已经开发出一种用于平行发光单细胞测定的微加工装置，该装置可以根据对刺激的动态功能反应对群体进行分类。Ibis装置是由一组规则排列的非接触单细胞阱组成。这些陷阱使用介电电泳来稳定地限制细胞，并保持它们不受干扰的流体流动。使用定量建模，我们设计了一种新的非对称挤出四极几何陷阱。这种新的陷阱可以物理排列和电寻址，使我们的细胞仪。在微通道中放置这些陷阱的阵列，我们已经将细胞引入阵列中，并展示了荧光动态响应的观察，然后进行分选。这样的设备有可能用于调查功能过程中，揭示了时间行为，在大量的单细胞。
We have developed a microfabricated device for use in parallel luminescent single-cell assays that can sort populations upon the basis of dynamic functional responses to stimuli. Ibis device is composed of a regular array of noncontact single-cell traps. These traps use dielectrophoresis to stably confine cells and hold them against disrupting fluid flows. Using quantitative modeling, we have designed traps with a novel asymmetric extruded-quadrupole geometry. This new trap can be physically arrayed and electrically addressed, enabling our cytometer. Situating an array of these traps in a microchannel, we have introduced cells into the array and demonstrated observation of fluorescent dynamic responses followed by sorting. Such a device has potential for use in investigating functional processes, as revealed by temporal behavior, in large numbers of single cells.