MKK7 confers different activities to viral infection of Singapore grouper iridovirus (SGIV) and nervous necrosis virus (NNV) in grouper.
MKK7 confers different activities to viral infection of Singapore grouper iridovirus (SGIV) and nervous necrosis virus (NNV) in grouper.
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MKK7对石斑鱼中新加坡石斑鱼虹彩病毒(SGIV)和神经坏死病毒(NNV)的病毒感染赋予不同的活性。
DOI：
10.1016/j.fsi.2016.09.002
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发表时间：
2016-10
期刊：
影响因子：
--
通讯作者：
Qin Q
中科院分区：
文献类型：
--
作者：
Guo M;Wei J;Zhou Y;Qin Q
丝裂原激活蛋白激酶 7 (MKK7) 是响应环境或生理刺激的主要应激激活蛋白激酶 (SAPK) 激活激酶之一。这里从橙斑石斑鱼 Epinephelus coioides 中鉴定出一个名为 Ec-MKK7 的 MKK7。 Ec-MKK7 的全长 cDNA 为 1853 bp，开放阅读框 (ORF) 为 1272 bp，编码 423 个氨基酸的推定蛋白。双特异性蛋白激酶、丝裂原激活蛋白激酶激酶 7 (PKc_MKK7) 的结构域中包含特征性的 S-K-A-K-T 基序。细胞内定位显示 Ec-MKK7 定位于石斑鱼脾 (GS) 和/或石斑鱼脑 (EAGB) 细胞的细胞质和细胞核中。此外，Ec-MKK7 在所有检查的组织中普遍表达，并且在体内对脂多糖 (LPS)、新加坡石斑鱼虹彩病毒 (SGIV) 和多核糖肌苷多核糖胞苷酸 (poly I:C) 的攻击表现出表达调节。采用过表达 Ec-MKK7 的基因打靶策略来检查 MKK7 对体外病毒感染的活性。我们的数据表明Ec-MKK7参与SGIV的逃避和复制，但对神经坏死病毒（NNV）的感染发挥抗病毒作用。所有结果均表明Ec-MKK7在石斑鱼天然免疫中发挥重要作用，并对病毒感染表现出独特的功能。
Mitogen-activated protein kinase 7 (MKK7) is one of the major stress-activated protein kinase (SAPK)-activating kinases in response to environmental or physiological stimuli. Here a MKK7 named as Ec-MKK7 was identified from orange-spotted grouper, Epinephelus coioides. The full-length cDNA of Ec-MKK7 was 1853 bp, with an open reading frame (ORF) of 1272 bp encoding a putative protein of 423 amino acids. A characteristic S-K-A-K-T motif was contained in the domain of dual-specificity protein kinase, mitogen-activated protein kinase kinase 7 (PKc_MKK7). Intracellular localization showed that Ec-MKK7 was localized in both the cytoplasm and the nucleus of grouper spleen (GS) and/or grouper brain (EAGB) cells. Moreover, Ec-MKK7 was universally expressed in all examined tissues and showed expression modulation to challenges of lipopolysacchride (LPS), Singapore grouper iridovirus (SGIV) and polyriboinosinic polyribocytidylic acid (poly I:C)in vivo. A gene targeting strategy over-expressing Ec-MKK7 was performed to examine the activities of MKK7 to viral infectionin vitro. Our data showed that Ec-MKK7 was involved in the evasion and replication of SGIV but played an antiviral role to the infection of nervous necrosis virus (NNV). All results demonstrated that Ec-MKK7 could play important roles in grouper innate immunity and show distinct functions on virus infection.
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影响因子：
5.7
作者：
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