Morphologic evidence for innervation of Deiters' and Hensen's cells in the guinea pig
Morphologic evidence for innervation of Deiters' and Hensen's cells in the guinea pig
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DOI：
10.1016/s0378-5955(97)00040-3
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发表时间：
1997-06-01
期刊：
影响因子：
2.8
通讯作者：
Nadol, JB
中科院分区：
文献类型：
--
作者：
Burgess, BJ;Adams, JC;Nadol, JB
应用突触素和神经丝的免疫染色、乙酰胆碱酯酶组织化学和透射电子显微镜观察成年豚鼠Corti器的Deiters细胞和Hensen细胞中神经纤维和末梢的存在。这些神经末梢似乎与戴特斯细胞和汉森细胞形成化学突触。与基底耳蜗相比，神经纤维和突触在顶端更常见。终末常出现在Hensen细胞的基部附属物上，这些附属物富含线粒体，通常含有高尔基体和致密的核心小泡。电子显微镜和神经丝免疫染色显示，大多数在顶端耳蜗Hensen的细胞接受神经支配。少数神经纤维和终末呈乙酰胆碱酯酶阳性，表明它们不是胆碱能橄榄耳蜗纤维的侧支。这些纤维的密度，如神经丝的免疫组化所示，远远大于以前的GABA能纤维的报告，这表明它们不是GABA能橄榄耳蜗纤维。这种纤维和突触与外毛细胞区的支持细胞的作用是未知的。这些纤维的起源和功能的确定将提供新的见解耳蜗的结构和功能。
The presence of nerve fibers and terminals among Deiters' and Hensen's cells of the organ of Corti of the adult guinea pig is demonstrated using immunostaining for synaptophysin and neurofilaments, acetylcholinesterase histochemistry, and transmission electron microscopy. These nerve terminals appeared to form chemical synapses with Deiters' and Hensen's cells. Nerve fibers and synapses were more common in the apical as compared to the basal cochlea. The terminals were often present on basal appendages of Hensen's cells, which were rich in mitochondria and often contained a Golgi apparatus and dense core vesicles. Electron microscopy and immunostaining for neurofilaments showed that most Hensen's cells in the apical cochlea received innervation. Few of the nerve fibers and terminals were positive for acetylcholinesterase, which suggests that they were not collaterals of cholinergic olivocochlear fibers. The density of these fibers, as shown by immunohistochemistry for neurofilaments, was far greater than previous reports of GABA-ergic fibers, which suggests that they were not GABA-ergic olivocochlear fibers. The role of such fibers and synapses with supporting cells of the outer hair cell area is unknown. Determination of the origins and functions of these fibers will provide new insights into cochlear structure and function.