3-DIMENSIONAL STRUCTURE OF ROTAVIRUS
3-DIMENSIONAL STRUCTURE OF ROTAVIRUS
复制标题
DOI：
10.1016/0022-2836(88)90313-0
复制
发表时间：
1988-01-20
影响因子：
5.6
通讯作者：
CHIU, W
中科院分区：
文献类型：
--
作者：
PRASAD, BVV;WANG, GJ;CHIU, W
双壳和单壳猿轮状病毒的三维结构已确定分辨率为 40 ANG。通过图像处理嵌入玻璃冰中未染色、未固定的病毒颗粒的电子显微照片。这项研究表明，这些结构中的二十面体表面晶格在左手配置中的三角剖分数为 13。双壳病毒颗粒具有光滑的外表面和 60 个细长的尖刺。相比之下，单壳病毒粒子呈现出刚毛状的表面。根据这些结构，推断出外壳蛋白和内壳蛋白的位置和拷贝数。这些尖峰可能对应于 VP3（一种血凝素），而外壳中的其余质量密度代表 780 个 VP7（一种中和抗原）分子。位于单壳病毒粒子的所有局部和严格的三重轴上的 260 个形态单元被认为代表 VP6 的 260 个三聚体，VP6 是一种亚组抗原。外壳和内壳之间的紧密接触区域主要位于局部且严格的三重轴附近。轮状病毒结构的一个显着特征是在连接最外层表面和内核的所有 5 个和 6 个协调位置上存在跨越两个壳的 132 个大通道。在具有转录活性的单壳病毒粒子中，这些通道可能提供输入病毒RNA转录所需的代谢物和输出新合成的RNA分子用于后续病毒复制过程的途径。
The three-dimensional structures of double and single-shelled simian rotavirus have been determined to a resolution of 40 .ANG. by image processing electron micrographs of unstained, unfixed virus particles embedded in vitreous ice. This study demonstrates that the icosahedral surface lattices in these structures have a triangulation number of 13 in a left-handed configuration. The double-shelled virion has a smooth outer surface with 60 slender spikes. The single-shelled virion, in contrast, exhibits a bristly surface. On the basis of these structures, the locations and number of copies of outer and inner shell proteins have been deduced. The spikes likely correspond to VP3, a hemagglutinin, while the rest of the mass density in the outer shell represents 780 molecules of VP7, a neutralization antigen. The 260 morphological units, located on all the local and strict 3-fold axes of the single-shelled virion are proposed to represent 260 trimers of VP6, which is a subgroup antigen. The regions of closed contact between the outer and the inner shells are located mainly near the local and strict 3-fold axes. A distinctive feature in the rotavirus structure is the presence of 132 large channels spanning across both the shells at all 5 and 6-co-ordinated positions linking the outermost surface with the inner core. In the transcriptionally active single-shelled virion, these channels may provide pathways for importing the metabolites required for the viral RNA transcription and exporting the newly synthesized RNA molecules for subsequent viral replication processes.