Connectivity map of bipolar cells and photo receptors in the mouse retina
Connectivity map of bipolar cells and photo receptors in the mouse retina
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DOI：
10.7554/elife.20041
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发表时间：
2016-11-25
期刊：
影响因子：
7.7
通讯作者：
Berens, Philipp
中科院分区：
文献类型：
--
作者：
Behrens, Christian;Schubert, Timm;Berens, Philipp
在小鼠视网膜中，三种不同类型的光感受器为14种双极细胞(BC)提供输入。经典地说，大多数BC类型被认为接触树突区域内的所有锥体；On-BCS将仅通过所谓的内陷突触接触锥体，而Off-BCS将形成基础突触。通过挖掘公开的电子显微镜数据，我们发现了有趣的违反这些外部视网膜连接规则的行为：On-BC X型只接触了树突区域中20%的视锥细胞，并形成了大多数非典型的非内陷接触。5T、50型和8型接触到的锥体也比预期的要少。此外，我们发现视杆BCS接受锥体的输入，这为视杆和视锥通路在两个方向上相互连接提供了解剖学证据。这表明，外丛状层的组织比经典认为的要复杂得多。
In the mouse retina, three different types of photoreceptors provide input to 14 bipolar cell (BC) types. Classically, most BC types are thought to contact all cones within their dendritic field; ON-BCs would contact cones exclusively via so-called invaginating synapses, while OFF-BCs would form basal synapses. By mining publically available electron microscopy data, we discovered interesting violations of these rules of outer retinal connectivity: ON-BC type X contacted only -20% of the cones in its dendritic field and made mostly atypical non-invaginating contacts. Types 5T, 5O and 8 also contacted fewer cones than expected. In addition, we found that rod BCs received input from cones, providing anatomical evidence that rod and cone pathways are interconnected in both directions. This suggests that the organization of the outer plexiform layer is more complex than classically thought.