Interactions Between the Yeast Ogataea pini and Filamentous Fungi Associated with the Western Pine Beetle
Interactions Between the Yeast Ogataea pini and Filamentous Fungi Associated with the Western Pine Beetle
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DOI：
10.1007/s00248-010-9773-8
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发表时间：
2011-04-01
影响因子：
3.6
通讯作者：
Keim, Paul
中科院分区：
文献类型：
--
作者：
Davis, Thomas S.;Hofstetter, Richard W.;Keim, Paul
除了丝状真菌之外，其他重要的微生物可以被安置在小蠹的真菌运输结构（mycangia）中。从美国亚利桑那州北方的西部松大小蠹（Dendroctonus brevicomis）种群的真菌囊中分离到酵母Ogataea pini（真菌目：松小蠹科），频率为56%。我们进行了一系列的体外试验，以测试是否挥发性有机化合物产生的O。pini影响了共生和拮抗丝状真菌的径向生长速率，这些丝状真菌通常与甲虫相关，包括Entomocorticium sp. B，Ophiostoma minus，Beauvaria bassiana和Aspergillus sp.。当生长在2%麦芽提取物琼脂上时，使用气相色谱/质谱法（GC/MS）分析顶空挥发物，O.在人工培养液中，pini显著促进了共生真菌Entomocorticium sp. B的生长，而抑制了昆虫病原真菌B的生长。巴西纳。GC/MS分析表明O.松树产生乙醇，二硫化碳（CS2），并在顶部空间δ-3-carene。这些研究结果暗示O. pini是D. brevicomis社区生态，我们介绍了多种假设，为未来的测试酵母菌的影响，共生组合与大小蠹树皮甲虫。
Ecologically important microbes other than filamentous fungi can be housed within the fungal-transport structures (mycangia) of Dendroctonus bark beetles. The yeast Ogataea pini (Saccharomycetales: Saccharomycetaceae) was isolated from the mycangia of western pine beetle (Dendroctonus brevicomis) populations in northern Arizona (USA) with a frequency of 56%. We performed a series of in vitro assays to test whether volatile organic compounds produced by O. pini affected radial growth rates of mutualistic and antagonistic species of filamentous fungi that are commonly found in association with the beetle including Entomocorticium sp. B, Ophiostoma minus, Beauvaria bassiana, and an Aspergillus sp. We determined the compounds O. pini produced when grown on 2% malt extract agar using a gas chromatography/mass spectrometry (GC/MS) analysis of headspace volatiles. Volatiles produced by O. pini on artificial media significantly enhanced the growth of the mutualistic Entomocorticium sp. B, and inhibited growth of the entomopathogenic fungus B. bassiana. GC/MS revealed that O. pini produced ethanol, carbon disulfide (CS2), and Delta-3-carene in headspace. The results of these studies implicate O. pini as an important component in D. brevicomis community ecology, and we introduce multiple hypotheses for future tests of the effects of yeasts in the symbiont assemblages associated with Dendroctonus bark beetles.