Mapping stem cell activities in the feather follicle
Mapping stem cell activities in the feather follicle
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DOI：
10.1038/nature04222
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发表时间：
2005-12-15
期刊：
影响因子：
64.8
通讯作者：
Chuong, CM
中科院分区：
文献类型：
--
作者：
Yue, ZC;Jiang, TX;Chuong, CM
了解不同器官如何“管理”它们的干细胞是很重要的。毛囊和羽囊都显示出强大的再生能力，可以间歇性地更新上皮器官。然而，羽毛（从爬行动物到鸟类）和毛发（从爬行动物到哺乳动物）的进化是独立的事件，它们的毛囊结构是趋同进化的结果。由于羽毛在解剖学上没有毛囊隆起的等价物，我们有兴趣确定它们的干细胞定位在哪里。通过应用长期标记保留（1），移植（2）和DiI示踪来绘制干细胞活性，在这里，我们发现羽毛毛囊含有慢循环的长期标记保留细胞（LRC），瞬时扩增细胞和分化的角质形成细胞。每个种群，位于解剖学上不同的区域，在羽毛周期中经历动态平衡。在生长卵泡中，LRC在“领突”龛中富集。在蜕皮卵泡中，LRC转移到真皮乳头附近的乳头状外胚层龛。在移植时，LRC表现出多潜能性。在三维视图中，LRC被配置为水平放置在径向对称羽毛中但倾斜放置在两侧对称羽毛中的环。干细胞活动的拓扑结构变化可能有助于复杂羽毛形式的构建。
It is important to know how different organs 'manage' their stem cells. Both hair and feather follicles show robust regenerative powers that episodically renew the epithelial organ. However, the evolution of feathers ( from reptiles to birds) and hairs ( from reptiles to mammals) are independent events and their follicular structures result from convergent evolution. Because feathers do not have the anatomical equivalent of a hair follicle bulge, we are interested in determining where their stem cells are localized. By applying long-term label retention(1), transplantation(2) and DiI tracing to map stem cell activities, here we show that feather follicles contain slow-cycling long-term label-retaining cells (LRCs), transient amplifying cells and differentiating keratinocytes. Each population, located in anatomically distinct regions, undergoes dynamic homeostasis during the feather cycle. In the growing follicle, LRCs are enriched in a 'collar bulge' niche. In the moulting follicle, LRCs shift to populate a papillar ectoderm niche near the dermal papilla. On transplantation, LRCs show multipotentiality. In a three-dimensional view, LRCs are configured as a ring that is horizontally placed in radially symmetric feathers but tilted in bilaterally symmetric feathers. The changing topology of stem cell activities may contribute to the construction of complex feather forms.