Involvement of small RNAs and role of the qde genes in the gene silencing pathway in Neurospora
Involvement of small RNAs and role of the qde genes in the gene silencing pathway in Neurospora
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DOI：
10.1101/gad.222402
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发表时间：
2002-04-01
影响因子：
10.5
通讯作者：
Cogoni, C
中科院分区：
文献类型：
--
作者：
Catalanotto, C;Azzalin, G;Cogoni, C
已发现小 RNA 分子与植物和动物中的转录后基因沉默 (PTGS) 特异性相关。在这里，我们发现小正义和反义RNA也参与粗糙脉孢菌的PTGS。这些RNA分子的积累取决于先前显示对基因沉默至关重要的功能性qde-1和qde-3基因的存在，但不依赖于功能性qde-2，表明该基因参与基因沉默途径的下游步骤。支持这一观点的是，纯化的 QDE2 蛋白复合物被发现含有小 RNA 分子，表明 QDE2 可能是参与序列特异性 mRNA 降解的小 RNA 指导的核糖核酸酶复合物的一部分。
Small RNA molecules have been found to be specifically associated with posttranscriptional gene silencing (PTGS) in both plants and animals. Here, we find that small sense and antisense RNAs are also involved in PTGS in Neurospora crassa. The accumulation of these RNA molecules depends on the presence of functional qde-1 and qde-3 genes previously shown to be essential for gene silencing, but does not depend on a functional qde-2, indicating that this gene is involved in a downstream step of the gene silencing pathway. Supporting this idea, a purified QDE2 protein complex was found to contain small RNA molecules suggesting that QDE2 could be part of a small RNA-directed ribonuclease complex involved in sequence-specific mRNA degradation.