Involvement of metal transporters in the intestinal uptake of cadmium
Involvement of metal transporters in the intestinal uptake of cadmium
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DOI：
10.2131/jts.45.539
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发表时间：
2020-01-01
影响因子：
2
通讯作者：
Ohba, Kenichi
中科院分区：
文献类型：
--
作者：
Ohta, Hisayoshi;Ohba, Kenichi
我们研究了正常情况下雌性大鼠肠道镉（Cd）摄取的机制，该机制基于铁、锌、铜和钙等必需元素的介质（金属转运体）。这些元素与从肠道摄取Cd相互作用。小肠各部位（十二指肠、空肠和回肠）的Cd浓度随Cd暴露量的增加而增加。然而。Cd浓度以十二指肠最高。ZIP14、DMT1和ATP7A基因的表达随Cd浓度的增加而增加。Cu浓度随Cd浓度的增加而降低。而随着Cd浓度的增加，Fe浓度呈下降趋势。ZIP14、DMT1和ATP7A基因表达水平与Cd浓度呈正相关。免疫组化染色显示，随着Cd浓度的升高，ZIP14和DMT1的阳性位点分散在十二指肠杯状细胞、可消化上皮细胞和固有层附近。Cd被诱导合成并结合金属硫蛋白（MT-I和-II）并在肠道组织中积累，主要是在十二指肠。这些发现表明，镉作为一种污染元素，可通过多种金属转运体（如Cu、Fe和Zn）从肠道中吸收。从而参与肠道Cd的吸收
We investigated the mechanism underlying intestinal cadmium (Cd) uptake based on the mediators (metal transporters) of essential elements, such as Fe, Zn, Cu, and Ca, under normal conditions in female rats. These elements interact with Cd uptake from the intestinal tract. Cd concentration at each site of the small intestine (duodenum, jejunum, and ileum) increased as Cd exposure increased. However. Cd concentration was the highest in the duodenum. The gene expression of ZIP14, DMT1, and ATP7A increased with increase in Cd concentration. Further, Cu concentration decreased as Cd concentration increased. In contrast Fe concentration displayed a decreasing tendency with the increase in Cd concentration. The gene expression levels of ZIP14, DMT1, and ATP7A were positively correlated with Cd concentration. Immunohistochemical staining revealed the positive sites of ZIP14 and DMT1 scattered in the area adjacent to the goblet cells, resoibable epithelial cells, and lamina propria in the duodenum tissue, according to the increase in Cd concentration. Cd is induced to synthesize and bind to metallothionein (MT-I and -II) and accumulate in the intestinal tissues, mainly in the duodenum. Such findings suggest that Cd, a contaminant element, is taken up from the intestinal tract by multiple metal transporters such as Cu, Fe, and Zn. thereby involving in the intestinal Cd absorption.