High-risk human papillomaviruses may have an important role in non-oral habits-associated oral squamous cell carcinomas in Taiwan
High-risk human papillomaviruses may have an important role in non-oral habits-associated oral squamous cell carcinomas in Taiwan
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DOI：
10.1309/c5p6nuq2nw6lctbp
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发表时间：
2003-12-01
影响因子：
3.5
通讯作者：
Chiang, CP
中科院分区：
文献类型：
--
作者：
Chang, JYF;Lin, MC;Chiang, CP
为探讨人乳头瘤病毒（HPV）在口腔癌发生中的作用，从103例口腔鳞状细胞癌（OSCC）和30例正常口腔粘膜（NOM）标本中提取DNA。巢式聚合酶链反应，DNA测序和基因芯片HPV分型被用来确定我们的样本中的多种HPV类型。我们发现，所有HPV类型和高危型HPV的阳性率在口腔鳞状细胞癌样本中明显较高（分别为49.5%和41.7%）（6/30 [20%; P < .01]和5/30 [17%; P <0.05]，在非口腔习惯（OH）相关的OSCC样本中显著更高（分别为31/51 [61%]和28/51 [55%]）比OH相关OSCC样本（分别为20/52 [38%; P < .05]和15/52 [29%; P < .001]）。高危型HPV和所有HPV的优势比分别为3.97（P = .0097）和3.92（P = .006）。我们的研究结果表明，HPV，特别是高危HPV，可能与OSCC的发展，特别是非OH相关的OSCC。
To evaluate the etiologic role of human papillomavirus (HPV) in oral carcinogenesis, DNA samples were purified from 103 oral squamous cell carcinoma (OSCC) and 30 normal oral mucosal (NOM) specimens. A nested polymerase chain reaction, DNA sequencing, and gene-chip HPV typing were used to identify multiple HPV types in our samples. We found that the positive rates of all HPV types and of high-risk HPV types were significantly higher in OSCC samples (49.5% and 41.7%, respectively) than in NOM samples (6/30 [20%; P < .01] and 5/30 [17%; P < .05], respectively) and significantly higher in non-oral habits (OH)-associated OSCC samples (31/51 [61%] and 28/51 [55%], respectively) than in OH-associated OSCC samples (20/52 [38%; P < .05] and 15/52 [29%; P < .001], respectively). High-risk HPV types and all HPV types had odds ratios of 3.97 (P = .0097) and 3.92 (P = .006), respectively. Our results suggest that HPVs, particularly high-risk HPVs, might be associated with the development of OSCCs, especially the non-OH-associated OSCCs.