GAMMA-INTERFERON INHIBITS COLLAGEN-SYNTHESIS INVIVO IN THE MOUSE
GAMMA-INTERFERON INHIBITS COLLAGEN-SYNTHESIS INVIVO IN THE MOUSE
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DOI：
10.1172/jci112945
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发表时间：
1987-04-01
影响因子：
15.9
通讯作者：
AMENTO, EP
中科院分区：
文献类型：
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作者：
GRANSTEIN, RD;MURPHY, GF;AMENTO, EP
将渗透泵皮下植入CAF 1小鼠导致在泵周围形成厚的纤维囊。当泵装载有重组鼠γ-干扰素（rMuIFN-γ）时，以递送2倍。103 U/h持续14天，来自rMuIFN-γ的胶囊的厚度和胶原含量显著降低。与来自仅接受稀释剂的动物的胶囊相比，通过对组织进行羟脯氨酸分析和对胶原束进行定量电子显微镜检查来估计胶囊的胶原含量。热灭活的rMuIFN-γ在该测定中未能降低纤维化反应。这些结果提供了令人信服的证据表明，γ-干扰素可以下调胶原蛋白的合成在体内，并建议的可能性，这种淋巴因子可能是有用的，在治疗疾病状态的特点是过度纤维化。
Subcutaneous implantation of osmotic pumps into CAF1 mice resulted in the formation of thick fibrous capsules around the pumps. When pumps were loaded with recombinant murine gamma-interferon (rMuIFN-.gamma.) to deliver 2 .times. 103 U/h for 14 d, there was a marked decrease in thickness and collagen content of the capsules from rMuIFN-.gamma.-treated animals compared with capsules from animals receiving diluent alone. The collagen content of the capsules was estimated by hydroxyproline analysis of the tissue and by quantitative electron microscopy of collagen bundles. Heat-inactivated rMuIFN-.gamma. failed to reduce the fibrotic response in this assay. These results provide compelling evidence that gamma-interferon can down-regulate collagen synthesis in vivo and suggest the possibility that this lymphokine may be useful in the treatment of disease states characterized by excessive fibrosis.