Polymerase chain reaction for rapid detection of Campylobacter jejuni in artificially contaminated foods
Polymerase chain reaction for rapid detection of Campylobacter jejuni in artificially contaminated foods
复制标题
DOI：
10.1046/j.1472-765x.1998.00411.x
复制
发表时间：
1998-09-01
影响因子：
2.4
通讯作者：
Slavik, MF
中科院分区：
文献类型：
--
作者：
Winters, DK;O'Leary, AE;Slavik, MF
空肠弯曲菌接种到一系列生食和即食食品中。这些食物样本被用作测试来源，以聚合酶链反应（PCR）检测这种细菌。具体检测营地。如159 bp的PCR产物所示，空肠的PCR产物对于所有预煮熟食切片和所测试的生家禽产品都是可能的。所有测试的蔬菜都与PCR检测兼容。哈密瓜、猕猴桃和菠萝检测呈阳性，而草莓、西瓜、葡萄和苹果检测呈阴性。通过使用巢式PCR检测，在122 bp处产生单一条带，西瓜和葡萄仅获得阳性结果，而苹果和草莓继续给出阴性反应。这些快速和特异性的检测坎普。空肠可与大部分食物配伍，确保食物安全。
Campylobacter jejuni was inoculated into a range of raw and ready-to-eat foods. These food samples were used as a test source for detection of this bacterium by the polymerase chain reaction (PCR). Specific detection of Camp. jejuni, as indicated by a PCR product of 159 bp, was possible with all pre-cooked deli-sliced and raw poultry products tested. All vegetables tested were compatible with the PCR assay. Cantaloupe, kiwi and pineapple tested positive while strawberries, watermelon, grapes and apples tested negative. By using a nested PCR assay that yields a single band at 122 bp, positive results mere obtained with watermelon and grapes while the apple and strawberries continued to give a negative reaction. These rapid and specific assays for Camp. jejuni are compatible with most foods in insuring the safety of the food product.