Suppression of macrophage responses to bacterial lipopolysaccharide by a non-secretory form of secretory leukocyte protease inhibitor
Suppression of macrophage responses to bacterial lipopolysaccharide by a non-secretory form of secretory leukocyte protease inhibitor
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DOI：
10.1016/s0167-4889(99)00111-1
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发表时间：
1999-09-21
影响因子：
5.1
通讯作者：
Ding, AH
中科院分区：
文献类型：
--
作者：
Zhu, J;Nathan, C;Ding, AH
分泌性白细胞蛋白酶抑制剂（SLPI）的表达抑制巨噬细胞对细菌脂多糖（LPS）的反应能力。在此，向细胞外培养基中加入重组或天然SLPI是非抑制性的，而用非分泌形式的SLPI转染是完全抑制性的，这是用干扰素-γ处理克服的效果。由未转染的巨噬细胞产生的SLPI的一部分定位于胞质溶胶中。因此，SLPI可以在细胞内起作用以阻断LPS对巨噬细胞的激活。(C)1999 Elsevier Science B. V.保留所有权利。
Expression of secretory leukocyte protease inhibitor (SLPI) suppresses the ability of macrophages to respond to bacterial lipoyolysaccharide (LPS). Here, addition of recombinant or native SLPI to the extracellular medium was non-suppressive, while transfection with a non-secretory form of SLPI was fully suppressive, an effect overcome by treatment with interferon-gamma. A portion of the SLPI produced by untransfected macrophages was localized in the cytosol. Thus, SLPI can act intracellularly to block macrophage activation by LPS. (C) 1999 Elsevier Science B.V. All rights reserved.