Electrospun nanofibers modified with phospholipid moieties for enzyme immobilization
Electrospun nanofibers modified with phospholipid moieties for enzyme immobilization
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DOI：
10.1002/marc.200600266
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发表时间：
2006-08-23
影响因子：
4.6
通讯作者：
Seta, Patrick
中科院分区：
文献类型：
--
作者：
Huang, Xiao-Jun;Xu, Zhi-Kang;Seta, Patrick
将含有磷脂侧部分的 PANCMPC 静电纺丝成平均直径为 90 nm 的纳米纤维。场发射扫描电镜用于表征纳米纤维的形态。这些磷脂修饰的纳米纤维由于具有优异的生物相容性、高比表面积和孔隙率等特点，有利于固定化酶的催化效率和活性，因此被探索作为酶固定化的载体。来自皱褶假丝酵母的脂肪酶通过吸附固定在这些纳米纤维上。初步结果表明，这些磷脂修饰的纳米纤维上的固定化脂肪酶的性能非常有前景。
PANCMPCs containing phospholipid side moieties were electrospun into nanofibers with a mean diameter of 90 nm. Field emission SEM was used to characterize the morphologies of the nanofibers. These phospholipid-modified nanofibers were explored as supports for enzyme immobilization due to the characteristics of excellent biocompatibility, high surface/volume ratio, and porosity, which were beneficial to the catalytic efficiency and activity of immobilized enzymes. Lipase from Candida rugosa was immobilized on these nanofibers by adsorption. Preliminary results indicated that the properties of the immobilized lipase on these phospholipid-modified nanofibers were greatly promising.