Dual-delivery of VEGF and PDGF by double-layered electrospun membranes for blood vessel regeneration
Dual-delivery of VEGF and PDGF by double-layered electrospun membranes for blood vessel regeneration
复制标题
双层电纺膜双重递送 VEGF 和 PDGF 用于血管再生
DOI：
10.1016/j.biomaterials.2012.12.005
复制
发表时间：
2013-03-01
期刊：
影响因子：
14
通讯作者：
Yuan, Xiaoyan
中科院分区：
文献类型：
--
作者：
Zhang, Hong;Jia, Xiaoling;Yuan, Xiaoyan
由于再狭窄和破裂，小直径血管的组织工程仍然是具有挑战性的。为了防止血栓形成，打算沿着移植物的管腔快速内皮化，然后在外部周围增殖血管平滑肌细胞（VSMC）以实现顺应性。为此，开发了两种改进的同轴静电纺丝技术来分别封装血管内皮生长因子（VEGF）和血小板衍生生长因子-bb（PDGF），以调节血管内皮细胞（VECs）和VSMCs的增殖。研究了双层电纺膜的释放特性、体外细胞增殖和体内植入情况，其独特之处在于双层电纺膜由内载VEGF的壳聚糖水凝胶/聚（乙二醇）-b-聚（L-丙交酯-己内酯）（PELCL）电纺膜和外载PDGF的乳液/PELCL电纺膜组成。结果发现，VEGF和PDGF的双重释放可促进血管内皮细胞在前6天的增殖，调节VSMC在前3天的缓慢增殖，而在第6天后使其快速增殖，这对血管再生非常有利。在体兔颈动脉移植4周后，血管移植物管腔和血管平滑肌细胞分别生长，未见血栓形成或破裂。结论：经修饰的电纺膜双重递送VEGF和PDGF可促进血管重建。(C)2013爱思唯尔有限公司保留所有权利。
Tissue engineering of small-diameter blood vessels is still challenging because of restenosis and burst To prevent thrombosis, rapid endothelialization along the lumen of grafts is intended, followed by proliferation of vascular smooth muscle cells (VSMCs) around the exterior for compliance. To this goal, two modified coaxial electrospinning techniques were developed to encapsulate vascular endothelial growth factor (VEGF) and platelet-derived growth factor-bb (PDGF), respectively, to regulate proliferation of vascular endothelial cells (VECs) and VSMCs. Release profiles, in vitro cell proliferation and in vivo implantation of double-layered electrospun membranes were investigated, and what made it special was the electrospun membranes were composed of chitosan hydrogel/poly(ethylene glycol)-b-poly(L-lactide-co-caprolactone) (PELCL) electrospun membrane loaded with VEGF as the inner layer and emulsion/PELCL electrospun membrane-loaded PDGF as the outer. It was found that dual-release of VEGF and PDGF could accelerate VEC proliferation in the first 6 days, and modulate slow VSMC proliferation in the initial 3 days whereas generate rapid proliferation after day 6, which is of great benefit to blood vessel regeneration. Four weeks of in vivo replacement of rabbit carotid artery demonstrated that VECs and VSMCs developed on the lumen and exterior of vascular grafts, respectively, and no thrombus or burst appeared. It was concluded that dual-delivery of VEGF and PDGF by the modified electrospun membranes could facilitate revascularization. (C) 2013 Elsevier Ltd. All rights reserved.