PATHOPHYSIOLOGY OF SMALL INTESTINAL MALABSORPTION IN GERBILS INFECTED WITH GIARDIA-LAMBLIA
PATHOPHYSIOLOGY OF SMALL INTESTINAL MALABSORPTION IN GERBILS INFECTED WITH GIARDIA-LAMBLIA
复制标题
DOI：
10.1016/0016-5085(92)90840-u
复制
发表时间：
1992-08-01
期刊：
影响因子：
29.4
通讯作者：
GALL, DG
中科院分区：
文献类型：
--
作者：
BURET, A;HARDIN, JA;GALL, DG
蒙古沙鼠感染人类致病性贾第鞭毛虫株，并与假治疗对照动物接种后6天。感染导致隐窝增生，肠上皮细胞迁移率增加。感染动物的十二指肠绒毛高度降低，空肠绒毛高度不变，回肠绒毛高度增加。感染动物空肠和回肠上皮微绒毛明显缩短，刷状缘表面积减少。胸苷激酶活性增加，在分离的十二指肠绒毛肠上皮细胞，但在空肠和回肠没有区别。在体外和体内实验表明，感染导致空肠葡萄糖刺激的电解质，水，和3-O-甲基-D-葡萄糖的吸收减少，而在回肠在体外电解质和3-O-甲基-D-葡萄糖的吸收是相似的感染和对照动物。因此，在空肠感染导致电解质，溶质，液体吸收不良与刷状缘表面积减少。结果表明，贾第虫病引起的腹泻是由吸收不良而不是主动分泌引起的。
Mongolian gerbils were infected with a human pathogenicGiardia lambliastrain and compared with sham-treated control animals 6 days after inoculation. Infection resulted in crypt hyperplasia associated with an increased enterocyte migration rate. Villus height was decreased in the duodenum, unchanged in the jejunum, and increased in the ileum of infected animals. Epithelial microvilli were markedly shortened, and brush border surface area decreased in the jejunum and ileum of infected animals. Thymidine kinase activity was increased in isolated duodenal villus enterocytes but did not differ in the jejunum and ileum. In vitro and in vivo experiments showed that the infection resulted in decreased jejunal glucose-stimulated electrolyte, water, and 3-O-methyl-d-glucose absorption, whereas in the ileum in vitro electrolyte and 3-O-methyl-d-glucose absorption was similar in infected and control animals. Thus, in the jejunum infection causes electrolyte, solute, and fluid malabsorption associated with decreased brush border surface area. The results indicate that the diarrhea associated with giardiasis is caused by malabsorption rather than active secretion.