Quantitative 3D analysis of complex single border cell behaviors in coordinated collective cell migration.
Quantitative 3D analysis of complex single border cell behaviors in coordinated collective cell migration.
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DOI：
10.1038/ncomms14905
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发表时间：
2017-04-04
影响因子：
16.6
通讯作者：
Yu W
中科院分区：
文献类型：
--
作者：
Cliffe A;Doupé DP;Sung H;Lim IK;Ong KH;Cheng L;Yu W
了解集体细胞迁移的机制对于癌症转移、伤口愈合和许多发育过程至关重要。在体内对迁移簇进行成像是可行的，但单个细胞行为的量化仍然具有挑战性。我们已经开发了一个图像分析工具包，CCMToolKit，以量化果蝇边缘细胞系统。除了混沌运动之外，先前的研究报道迁移细胞能够以高度协调的模式迁移。我们在3D中量化了旋转和运行迁移模式，同时还观察了一系列中间行为。运行模式是由集群外部突起驱动。旋转模式与不易表征的群集内部单元扩展相关。虽然集群在旋转时移动较慢，但单个单元保持其移动性，实际上比运行模式下稍微更活跃。我们还表明，个别细胞可能会在迁移过程中交换位置。量化体内细胞行为对于理解集体细胞迁移的机制至关重要。在这里，作者提出了一个图像分析工具包，CCMToolKit，描述和表征各种模式的协调细胞运动伴随着集体细胞迁移果蝇边缘细胞。
Understanding the mechanisms of collective cell migration is crucial for cancer metastasis, wound healing and many developmental processes. Imaging a migrating cluster in vivo is feasible, but the quantification of individual cell behaviours remains challenging. We have developed an image analysis toolkit, CCMToolKit, to quantify the Drosophila border cell system. In addition to chaotic motion, previous studies reported that the migrating cells are able to migrate in a highly coordinated pattern. We quantify the rotating and running migration modes in 3D while also observing a range of intermediate behaviours. Running mode is driven by cluster external protrusions. Rotating mode is associated with cluster internal cell extensions that could not be easily characterized. Although the cluster moves slower while rotating, individual cells retain their mobility and are in fact slightly more active than in running mode. We also show that individual cells may exchange positions during migration. Quantifying cell behaviours in vivo is essential to understanding the mechanisms of collective cell migration. Here the authors present an image analysis toolkit, CCMToolKit, to describe and characterize various modes of coordinated cell movements accompanying collective cell migration in Drosophila border cells.