Plasmonic enhancement of cyanine dyes for near-infrared light-triggered photodynamic/photothermal therapy and fluorescent imaging
Plasmonic enhancement of cyanine dyes for near-infrared light-triggered photodynamic/photothermal therapy and fluorescent imaging
复制标题
用于近红外光触发光动力/光热治疗和荧光成像的花青染料的等离子体增强
DOI：
10.1088/1361-6528/aa81e1
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发表时间：
2017-11-03
期刊：
影响因子：
3.5
通讯作者：
Wang, Xiumin
中科院分区：
文献类型：
--
作者：
Lu, Mindan;Kang, Ning;Wang, Xiumin
近红外（NIR）触发的花青染料在多模式肿瘤治疗诊断学中引起了相当大的关注。然而，用于肿瘤治疗的NIR花青染料通常具有低荧光强度和弱单线态氧产生效率，导致对肿瘤的诊断和治疗功效不足。提高花菁染料在肿瘤光动力学治疗和荧光成像中的应用效果仍然是一个巨大的挑战。本文基于金属增强荧光（MEF）效应，开发了一种新型多功能纳米药物AuNRs@SiO2-IR 795，以实现光热/光动力综合治疗（PTT/PDT）和FLI。在我们的设计中，AuNRs@SiO2-IR 795纳米复合材料的荧光强度和活性氧物种与游离IR 795相比显著增强高达51.7和6.3倍，这是由于AuNRs的局部表面等离子体共振带与IR 795染料的吸收或荧光发射带重叠。在近红外激光照射下，PDT/PTT协同作用对肿瘤细胞的体外抑制率高达82.3%，而游离IR 795的抑制率仅为10.3%。此外，我们设计的纳米复合材料的增强的荧光强度有助于跟踪它们在肿瘤细胞中的行为。因此，我们设计的纳米药物突出了基于MEF的肿瘤多模式诊断和治疗的应用。
Near-infrared (NIR) triggered cyanine dyes have attracted considerable attention in multimodal tumor theranostics. However, NIR cyanine dyes used in tumor treatment often suffer from low fluorescence intensity and weak singlet oxygen generation efficiency, resulting in inadequate diagnostic and therapy efficacy for tumors. It is still a great challenge to improve both the photodynamic therapy (PDT) and fluorescent imaging (FLI) efficacy of cyanine dyes in tumor applications. Herein, a novel multifunctional nanoagent AuNRs@SiO2-IR795 was developed to realize the integrated photothermal/photodynamic therapy (PTT/PDT) and FLI at a very low dosage of IR795 (0.4 μM) based on metal-enhanced fluorescence (MEF) effects. In our design, both the fluorescence intensity and reactive oxygen species of AuNRs@SiO2-IR795 nanocomposites were significantly enhanced up to 51.7 and 6.3 folds compared with free IR795, owing to the localized surface plasmon resonance band of AuNRs overlapping with the absorption or fluorescence emission band of the IR795 dye. Under NIR laser irradiation, the cancer cell inhibition efficiency in vitro with synergetic PDT/PTT was up to 82.3%, compared with 10.3% for free IR795. Moreover, the enhanced fluorescence intensity of our designed nanocomposites was helpful to track their behavior in tumor cells. Therefore, our designed nanoagents highlight the applications of multimodal diagnostics and therapy in tumors based on MEF.