Real-time mapping of a hydrogen peroxide concentration profile across a polymicrobial bacterial biofilm using scanning electrochemical microscopy
Real-time mapping of a hydrogen peroxide concentration profile across a polymicrobial bacterial biofilm using scanning electrochemical microscopy
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DOI：
10.1073/pnas.1018391108
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发表时间：
2011-02-15
影响因子：
11.1
通讯作者：
Bard, Allen J.
中科院分区：
文献类型：
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作者：
Liu, Xiuhui;Ramsey, Matthew M.;Bard, Allen J.
通过扫描电化学显微镜 (SECM) 实时研究戈登链球菌 (Sg) 细菌生物膜上方溶液中过氧化氢的定量检测。在 10 mM 葡萄糖存在下，经过 2 至 8 小时，过氧化氢的浓度测定为 0.7 mM 至 1.6 mM。与浮游生长的 Sg 相比，生物膜表面附近测得的过氧化氢产量更高。通过荧光测定和 SECM 测量观察过氧化氢产量的差异。 SECM 还研究了 Sg 细菌生物膜和 Aggregatibacter actinomycetemcomitans (Aa) 中两种不同物种在过氧化氢产生方面的相互作用。单向 y 扫描 SECM 测量显示了混合物种生物膜上过氧化氢浓度的独特空间映射，并揭示了过氧化氢浓度在很大程度上取决于当地物种组成。
Quantitative detection of hydrogen peroxide in solution above a Streptococcus gordonii (Sg) bacterial biofilm was studied in real time by scanning electrochemical microscopy (SECM). The concentration of hydrogen peroxide was determined to be 0.7 mM to 1.6 mM in the presence of 10 mM glucose over a period of 2 to 8 h. The hydrogen peroxide production measured was higher near the biofilm surface in comparison to Sg grown planktonically. Differential hydrogen peroxide production was observed both by fluorometric as well as by SECM measurements. The interaction between two different species in a bacterial biofilm of Sg and Aggregatibacter actinomycetemcomitans (Aa) in terms of hydrogen peroxide production was also studied by SECM. One-directional y-scan SECM measurements showed the unique spatial mapping of hydrogen peroxide concentration across a mixed species biofilm and revealed that hydrogen peroxide concentration varies greatly dependent upon local species composition.