Sex differences in urocortin 1 dynamics in the non-preganglionic Edinger-Westphal nucleus of the rat
Sex differences in urocortin 1 dynamics in the non-preganglionic Edinger-Westphal nucleus of the rat
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DOI：
10.1016/j.neures.2009.10.003
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Kozicz, L. Tamas
中科院分区：
文献类型：
--
作者：
Derks, Nicole M.;Gaszner, Balazs;Kozicz, L. Tamas
妇女比男子更容易受到压力和压力引起的疾病的伤害。雌激素可能通过雌激素受体α和β (ER α / β)参与性别依赖性应激适应的控制。npEW中的Urocortin 1 （Ucn1）在应激适应中起重要作用。我们假设npEW-Ucn1神经元的活性在两性之间是不同的，并且与雌激素信号有关。我们在这里通过免疫细胞化学表明，在npEW-Ucn1神经元中缺乏ER α而存在ER β。npEW的Q-RT-PCR证实了这一观点，表明雄性大鼠的ER β mRNA比雌性大鼠高出近5倍。此外，在发情后期和发情前期，雄性的Ucn1 mRNA分别比雌性高近10倍和1.6倍，表明Ucn1生物合成活性存在性别依赖性差异。同时，由于免疫细胞化学显示，在npEW-Ucn1神经元细胞体中储存的Ucn1肽的数量在雄性和雌性之间没有差异，根据这些神经元的数量和免疫信号密度来判断，我们提出轴突Ucn1运输的速度，可能还有Ucn1分泌的强度，与Ucn1生物合成的程度相同，依赖于性别。(C) 2009爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
Women have higher vulnerability to stress and stress-induced diseases than men. Estrogen may be involved in the control of sex-dependent stress adaptation via estrogen receptors alpha and beta (ER alpha/beta). Urocortin 1 (Ucn1) in the npEW plays an important role in stress adaptation. We hypothesize that the activity of npEW-Ucn1 neurons differs between sexes and is related to estrogen signalling. We here indicate by immunocytochemistry the absence of ER alpha and the presence of ER beta in the npEW-Ucn1 neurons. Q-RT-PCR of the npEW confirmed this notion, demonstrating that in male rats ER beta mRNA was almost 5 times higher than in females in di-estrus. Furthermore, Ucn1 mRNA in males was nearly 10 times and 1.6 times higher than in females in di-and pro-estrus, respectively, indicating a sex-dependent difference in Ucn1 biosynthetic activity. Since, at the same time, immunocytochemistry revealed that the amount of Ucn1 peptide stored in the cell bodies of the npEW-Ucn1 neurons did not differ between males and females, as judged on the basis of the number and immunosignal density of these neurons, we propose that the rate of axonal Ucn1 transport and, possibly, the strength of Ucn1 secretion, are dependent on sex to the same degree as is Ucn1 biosynthesis. (C) 2009 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.