Toward Safe Genetically Modified Organisms through the Chemical Diversification of Nucleic Acids
Toward Safe Genetically Modified Organisms through the Chemical Diversification of Nucleic Acids
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DOI：
10.1002/cbdv.200900083
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发表时间：
2009-01-01
影响因子：
2.9
通讯作者：
Marliere, Philippe
中科院分区：
文献类型：
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作者：
Herdewijn, Piet;Marliere, Philippe
有人认为，遗传增殖应该合理扩展，以便能够在体内繁殖其他类型的核酸（XNA 表示“异种核酸”），其化学骨架基序将不同于脱氧核糖和核糖，并且其聚合不会干扰 DNA 和 RNA 生物合成。因为 XNA 构建块不会在自然界中发生，所以它们必须被合成并提供给配备有所有适当酶促机器的细胞。用于聚合它们。从长远来看，XNA 复制子对植物和动物的入侵是可以预见的，但正是病态微生物，更具体地说是病态细菌，这种化学系统的可行性以及与 DNA 和 RNA 分离的遗传飞地的建立更有可能发生。基于替代主链基序和离去基团的复制子的繁殖将促进通过额外或替代配对引入扩展编码，正如为此目的有目的地进化的 XNA 聚合酶所实现的那样。
It is argued that genetic proliferation should be rationally extended so as to enable the propagation in vivo of additional types of nucleic acids (XNA for 'xeno-nucleic acids'), Whose Chemical backbone motifs would differ from deoxyribose and ribose, and whose polymerization would not interfere with DNA and RNA biosynthesis. Because XNA building blocks do not occur ill nature, they would have to be synthesized and Supplied to cells which would be equipped with ail appropriate enzymatic machinery. for polymerizing them. The invasion of plants and animals with XNA replicons call lie envisioned in the long run, but it is ill microorganisms, and more specifically ill bacteria, that the feasibility of such chemical systems and the establishment of genetic enclaves separated from DNA and RNA is more likely to take place. The introduction of expanded coding through additional or alternative pairing will be facilitated by the propagation of replicons based on alternative backbone motifs and leaving groups, as enabled by XNA polymerases purposefully evolved to this end.