THE SPECIFICITY OF ANTIBODIES TO THE F(AB')2 FRAGMENT OF HUMAN-IGG
THE SPECIFICITY OF ANTIBODIES TO THE F(AB')2 FRAGMENT OF HUMAN-IGG
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DOI：
10.1002/art.1780280516
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发表时间：
1985-01-01
影响因子：
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通讯作者：
ABRUZZO, JL
中科院分区：
文献类型：
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作者：
HEIMER, R;WOLFE, LD;ABRUZZO, JL
在类风湿关节炎患者的血清和亲和纯化的抗体制剂中检测了抗F(ab‘)2抗体的特异性。用凝集的人F(ab‘)2片段吸附在微滴板上，用酶联免疫吸附试验对血清进行检测，结果表明，Ig G抗F(ab’)2抗体可与人、兔Ig G和兔F(ab‘)2交叉反应。用亲和层析纯化的Ig G抗F(ab’)2抗体，经液相抑制酶联免疫吸附试验鉴定为2种类型。其中一组分类似于胃酶凝集剂，仅与人F(ab‘’)2片段反应。另一组分与人免疫球蛋白有交叉反应，但不与Fab上的独特位或Fc片段上的表位反应。纯化的Ig G抗F(ab‘)2抗体与由破伤风类毒素和类抗毒素制备的人免疫复合物无反应。
The specificity of IgG anti-F(ab'')2 antibodies was examined in unfractionated sera of patients with rheumatoid arthritis and also with affinity-purified antibody preparations. Examination of the sera by an enzyme-linked immunosorbent assay, using pooled human F(ab'')2 fragments absorbed to microtiter plates, revealed that IgG anti-F(ab'')2 antibodies cross-react with human and rabbit IgG and rabbit F(ab'')2. IgG anti-F(ab'')2 antibodies were purified by affinity chromatography and, when tested by a fluid-phase inhibition enzyme-linked immunosorbent assay, were found to be of 2 types. One fraction, similar to pepsin agglutinator, reacted with human F(ab'')2 fragment alone. The other fraction was cross-reactive with human IgG and yet failed to react with idiotopes on Fab or epitopes on Fc fragments. The IgG anti-F(ab'')2 antibodies purified had no reactivity toward a human immune complex prepared from tetanus toxoid and antitoxoid.