Is there a code for protein-DNA recognition? Probab(ilistical)ly ...
Is there a code for protein-DNA recognition? Probab(ilistical)ly ...
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DOI：
10.1002/bies.10073
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发表时间：
2002-05-01
期刊：
影响因子：
4
通讯作者：
Stormo, GD
中科院分区：
文献类型：
--
作者：
Benos, PV;Lapedes, AS;Stormo, GD
所有基因的转录调控都是由称为转录因子的调节蛋白与称为启动子区的DNA上的特定位点特异性结合而启动的。转录因子采用多种机制来识别它们的DNA靶位点。在过去的几十年里，人们试图通过通用的规则集和相关的模型来描述这些机制。我们给出了这些模型的概述，从一个有点争议的问题的“识别代码”管理蛋白质-DNA相互作用的历史回顾。然后，我们提出了一个概率框架，在其中可以讨论各种模型的优点和缺点。最后，我们的结论是，简化假设的相互作用的加和性是充分合理的，在许多情况下（并可以适当地扩展到其他情况下），允许一个统一的概念的“概率码”，蛋白质-DNA识别的定义。(C)2002 Wiley Periodicals，Inc.
Transcriptional regulation of all genes is initiated by the specific binding of regulatory proteins called transcription factors to specific sites on DNA called promoter regions. Transcription factors employ a variety of mechanisms to recognise their DNA target sites. In the last few decades, attempts have been made to describe these mechanisms by general sets of rules and associated models. We give an overview of these models, starting with a historical review of the somewhat controversial issue of a "recognition code" governing protein-DNA interaction. We then present a probabilistic framework in which advantages and disadvantages of various models can be discussed. Finally, we conclude that simplifying assumptions about additivity of interactions are sufficiently justified in many situations (and can be suitably extended in other situations) to allow a unifying concept of a "probabilistic code", for protein-DNA recognition to be defined. (C) 2002 Wiley Periodicals, Inc.