Analysis of phosphorylated isoforms of the p53 tumor suppressor protein in human lung carcinoma cells undergoing apoptosis.
Analysis of phosphorylated isoforms of the p53 tumor suppressor protein in human lung carcinoma cells undergoing apoptosis.
复制标题
人肺癌细胞凋亡过程中 p53 肿瘤抑制蛋白磷酸化亚型的分析。
DOI：
10.1002/elps.1150171115
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发表时间：
1996
期刊：
影响因子：
2.9
通讯作者：
Mukhopadhyay,T
中科院分区：
文献类型：
--
作者：
Maxwell,SA;Roth,JA;Mukhopadhyay,T
采用双向凝胶电泳技术研究了2-甲氧基乙烯诱导的H460 a细胞凋亡中p53肿瘤抑制蛋白亚型的表达。通过二维电泳分离来自对照和凋亡H460 a细胞的全细胞蛋白，并转移至硝酸纤维素。使用p53单克隆抗体Bp 53 - 12通过免疫印迹法检测p53亚型。在对照细胞中检测到磷酸化状态不同的四种p53亚型（2，3，5和6）。观察到另外三种亚型（1、4和7）仅在凋亡细胞中以显著水平表达。亚型1，4和7在凋亡细胞中的差异表达表明，产生这些形式的p53的一个或多个特定的磷酸化事件可能在调节p53在凋亡中的功能中发挥作用。
The expression of p53 tumor suppressor protein isoforms in H460a cells induced to undergo apoptosis by 2‐methoxyestradiol was investigated by two‐dimensional gel electrophoresis. Whole‐cell proteins from control and apoptotic H460a cells were separated by two‐dimensional electrophoresis and were transferred to nitrocellulose. The p53 isoforms were detected by immunoblotting using p53 monoclonal antibody Bp53‐12. Four isoforms of p53 (2, 3, 5, and 6) differing in phosphorylation state were detected in control cells. Three additional isoforms (1, 4, and 7) were observed to be expressed at significant levels only in apoptotic cells. The differential expression of isoforms 1, 4, and 7 in apoptotic cells suggests that one or more specific phosphorylation events generating these forms of p53 could play a role in regulating the function of p53 in apoptosis.