Folate production by bifidobacteria as a potential probiotic property
Folate production by bifidobacteria as a potential probiotic property
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DOI：
10.1128/aem.01763-06
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发表时间：
2007-01-01
影响因子：
4.4
通讯作者：
Rossi, Maddalena
中科院分区：
文献类型：
--
作者：
Pompei, Anna;Cordisco, Lisa;Rossi, Maddalena
对76株双歧杆菌产叶酸的能力进行了研究。为了评估叶酸生产力，在不含叶酸的半合成培养基SM 7中培养双歧杆菌。大多数测试菌株需要叶酸来生长。维生素的产生和积累程度不是物种的函数，而是个体菌株的显着特征。在17株不含叶酸的菌株中，有6株产生的维生素浓度明显较高（在41至82 ng/ml之间）。外源叶酸和对氨基苯甲酸（PABA）浓度对叶酸生产的影响进行了评估。与大多数其它菌株相比，B的叶酸产量高于其它菌株。PABA或外源性叶酸均未对拟南芥MB 239产生负面影响。由B产生叶酸。在结肠环境的pH范围内研究了大肠杆菌MB 239，并比较了属于三组重要的可发酵肠碳源的分枝糖、乳糖和低聚果糖的叶酸产量。B生物合成叶酸的差异。没有观察到作为pH或碳源的函数的羧甲基纤维素MB 239。粪培养实验表明，添加B.叶酸MB 239可能会增加结肠环境中的叶酸浓度。
The ability of 76 Bifidobacterium strains to produce folate was investigated. In order to evaluate folic acid productivity, bifidobacteria were cultivated in the folate-free semisynthetic medium SM7. Most of the tested strains needed folate for growth. The production and the extent of vitamin accumulation were not a function of species but were distinctive features of individual strains. Six strains among the 17 that grew without folate produced significantly higher concentrations of vitamin (between 41 and 82 ng ml(-1)). The effects of exogenous folate and p-aminobenzoic acid (PABA) concentrations on folate production were evaluated. In contrast to most of the other strains, the folate yield of B. adolescentis MB 239 was not negatively affected by either PABA or exogenous folic acid. Folate production by B. adolescentis MB 239 was studied in the pH range of the colonic environment, and a comparison of folate production on ramose, lactose, and fructo-oligosaccharides, which belong to three important groups of fermentable intestinal carbon sources, was established. Differences in folate biosynthesis by B. adolescentis MB 239 were not observed as a function either of the pH or of the carbon source. Fecal culture experiments demonstrated that the addition of B. adolescentis MB 239 may increase the folate concentration in the colonic environment.