Diseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay
Diseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay
复制标题
DOI：
10.1007/s42770-019-00170-7
复制
发表时间：
2019-10-24
影响因子：
2.2
通讯作者：
Giannitti, Federico
中科院分区：
文献类型：
--
作者：
da Silva Silveira, Caroline;Maya, Leticia;Giannitti, Federico
牛病毒性腹泻病毒（BVDV，Pestivirus）在世界范围内给畜牧业造成重大经济损失。尽管血清学调查显示，BVDV暴露在乌拉圭的牛中广泛存在，但BVDV相关疾病的报告严重不足。这项工作的目的是描述乌拉圭牛BVDV相关疾病自发暴发的流行病学、临床、病理学和病毒学结果。2016年11月至2018年4月期间，在科洛尼亚、里奥内格罗和索里亚诺省的肉牛和奶牛场发生的6起自发性疾病暴发期间进行了诊断调查。对这些暴发中的8头自然死亡牛的尸体进行尸检，并进行组织学检查和免疫组织化学，以检测组织中的BVDV抗原。还进行了逆转录实时PCR和基因组测序，以鉴定种属和亚型水平的BVDV。其他辅助诊断试验，包括细菌培养，是在个案的基础上进行，以排除在/出基于初步临床病理学推定诊断的鉴别诊断。在8例病例中诊断的BVDV相关疾病包括粘膜疾病、与鼠伤寒沙门氏菌引起的消化道/败血症沙门氏菌病相关的一过性产后BVDV感染、睡眠嗜组织菌支气管肺炎、大肠埃希菌和链球菌属的尿路合并感染，肠内共感染球虫，经胎盘胎儿感染和流产与新孢子虫犬共感染。在8例病例中的4例中分别鉴别出BVDV-1a和BVDV-2b。我们得出结论，BVDV-1a和BVDV-2b对乌拉圭牛的疾病和死亡率有显著影响。未来研究应估计BVDV对乌拉圭畜牧业的经济影响。
Bovine viral diarrhea virus (BVDV, Pestivirus) causes significant economic losses to the livestock industry worldwide. Although serological surveys show that BVDV exposure is widespread in cattle in Uruguay, BVDV-associated diseases are greatly underreported. The aim of this work is to describe the epidemiological, clinical, pathological, and virological findings from spontaneous outbreaks of BVDV-associated diseases in cattle in Uruguay. Diagnostic investigations were performed during 6 spontaneous disease outbreaks on beef and dairy cattle farms in the departments of Colonia, Rio Negro, and Soriano between November 2016 and April 2018. Carcasses of 8 naturally deceased cattle from these outbreaks were necropsied and subjected to histological examination and immunohistochemistry to detect BVDV antigen in the tissues. Reverse transcription real-time PCR and genomic sequencing were also performed to identify BVDV at the species and subtype levels. Other ancillary diagnostic tests, including bacterial cultures, were performed on a case-by-case basis to rule in/out differential diagnoses based on initial clinicopathological presumptive diagnoses. BVDV-associated conditions that were diagnosed in the 8 cases included mucosal disease, transient postnatal BVDV infections associated with digestive/septicemic salmonellosis by Salmonella serovar typhimurium, Histophilus somni bronchopneumonia, urinary tract coinfections with Escherichia coli and Streptococcus sp., enteric coinfection with coccidia, and transplacental fetal infections and abortions with Neospora caninum coinfection. BVDV-1a and BVDV-2b were each identified in four of the eight cases. We conclude that BVDV-1a and BVDV-2b contribute significantly to disease and mortality in cattle in Uruguay. Future research should estimate the economic impact of BVDV in the Uruguayan livestock sector.