Structural characterization of a galactan from Dioscorea opposita Thunb. and its bioactivity on selected Bacteroides strains from human gut microbiota
Structural characterization of a galactan from Dioscorea opposita Thunb. and its bioactivity on selected Bacteroides strains from human gut microbiota
复制标题
薯蓣半乳聚糖的结构表征。
DOI：
10.1016/j.carbpol.2019.04.084
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发表时间：
2019
影响因子：
11.2
通讯作者：
Kan Ding
中科院分区：
文献类型：
--
作者：
Chun-Qian Zhang;Fei He;Xia Chen;Kan Ding
野牡丹具有调理脾胃的功效，在我国被广泛用作功能食品和中药。多糖可能有助于调节功能。目的研究山茱萸多糖的结构特征和生物活性。opposita，D.用沸水浸提，得粗多糖DOP。从DOP中分离得到一种新的多糖DOP0.1-S-1。DOP 0.1-S-1的平均分子量为10，000 Da，分子量范围为12，000 - 1，200 Da，糖含量为100%，未检测到蛋白质。单糖分析表明，DOP 0.1-S-1主要由半乳糖组成。甲基化和NMR分析表明DOP 0.1-S-1为1，4-β-半乳聚糖。生物活性试验表明，DOP 0.1-S-1对B.太奥陶米克罗和B.在利用多糖的过程中产生短链脂肪酸。
Dioscorea oppositaThunb. is widely used as functional foods and traditional Chinese medicine in China for its activity of regulating function of spleen and stomach. Polysaccharides may contribute to the function of regulation. To investigate structure features and bioactivities of polysaccharides fromD. opposita,the rhizome ofD. oppositawas extracted with boiling water, yielding crude polysaccharides DOP. A novel polysaccharide named DOP0.1-S-1 was isolated from DOP by further purification. The average molecular weight of DOP0.1-S-1 was 10,000 Da and the range was around 12,000 –1,200 Da. The carbohydrate content of DOP0.1-S-1 was 100% and no protein was detected. The monosaccharide analysis showed that DOP0.1-S-1 was mostly composed of galactose. Methylation and NMR spectra analysis indicated that DOP0.1-S-1 was a 1,4-β-galactan. Bioactivity test showed that DOP0.1-S-1 could promote the growth ofB. thetaiotaomicronandB. ovatusand produce the short-chain fatty acids during the utilization of the polysaccharide.