Amperometric biosensor for NADH and ethanol based on electroreduced graphene oxide-polythionine nanocomposite film
Amperometric biosensor for NADH and ethanol based on electroreduced graphene oxide-polythionine nanocomposite film
复制标题
基于电还原氧化石墨烯-聚硫氨酸纳米复合膜的NADH和乙醇电流生物传感器
DOI：
10.1016/j.snb.2013.01.072
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发表时间：
2013-05-01
影响因子：
8.4
通讯作者：
Yao, Shouzhuo
中科院分区：
文献类型：
--
作者：
Li, Zou;Huang, Yi;Yao, Shouzhuo
采用循环伏安法在玻碳电极上制备了氧化石墨烯(ERGO)和聚硫醚(PTH)纳米复合膜。通过电化学石英晶体微天平(EQCM)方法研究了ERGO-PTH薄膜的电沉积过程，以便更深入地了解这一过程。将ERGO-PTH纳米复合薄膜用作促进NADH电催化氧化的传感器，并构建了基于脱氢酶的安培型生物传感器。在最佳条件下，NADH的安培检测具有线性范围宽(0.01~3.9 mm)、高灵敏度(143mA mm(-1)cm(-2))和低检测限(LOD=0.1mM，S/N=3)的特点。以乙醇脱氢酶为模型，研究了乙醇脱氢酶修饰的ERGO-PTH/GC电极对乙醇浓度的线性响应，灵敏度为2.8mA mm~(-1)cm~(-2)，检出限为0.3mU M(S/N=3)。(C)2013爱思唯尔B.V.保留所有权利。
A nanocomposite film is prepared by cyclic voltammetric deposition of electroreduced graphene oxide (ERGO) and polythionine (PTH) on a glassy carbon (GC) electrode. The electrodeposition of ERGO-PTH film is also investigated via the electrochemical quartz crystal microbalance (EQCM) method for more in-depth understanding of the process. The ERGO-PTH nanocomposite film is applied as transducer for facilitated electrocatalytic oxidation of NADH and for the construction of dehydrogenase-based amperometric biosensor. Under optimum conditions, the amperometric detection of NADH provides a wide linear detection range (0.01-3.9 mM), a high sensitivity (143 mu A mM(-1) cm(-2)) and a low limit of detection (LOD = 0.1 mu M, S/N = 3). With alcohol dehydrogenase (ADH) as a model, we obtain a linear amperometric response of the ADH-modified ERGO-PTH/GC electrode to ethanol concentration from 0.05 to 1.0 mM, a sensitivity of 2.8 mu A mM(-1) cm(-2), and a LOD of 0.3 mu M (S/N = 3). (C) 2013 Elsevier B.V. All rights reserved.