In Vivo, In Situ Tissue Analysis Using Rapid Evaporative Ionization Mass Spectrometry
In Vivo, In Situ Tissue Analysis Using Rapid Evaporative Ionization Mass Spectrometry
复制标题
DOI：
10.1002/anie.200902546
复制
发表时间：
2009-01-01
影响因子：
16.6
通讯作者：
Takats, Zoltan
中科院分区：
文献类型：
--
作者：
Schaefer, Karl-Christian;Denes, Julia;Takats, Zoltan
通过质谱（MS）分析完整生物组织已经进行了三十多年。然而，质谱方法总是对这些样品的几何形状和制备施加强约束。即使最近出现了环境电离方法，也不是所有这些限制都被取消了。[1-5]组织中生物分子的MS分析传统上通过解吸电离方法实现，包括二次离子质谱（西姆斯），[6-11]基质辅助激光解吸（MALDI），[12-17，19，20]和解吸电喷雾电离（DESI）[4，5，18]方法。虽然解吸电离方法不适合于分析重要的（活的）组织，但快速热蒸发有可能建立组织成分的原位体内电离。Holland等人最初提出了在纯粹的热过程中从凝聚相样品中可能形成有机离子，[21]后来成功地演示了。[22-24]快速加热的基本原理是实现与分解速率相当的分子蒸发速率，这导致形成大量的气态分子或分子离子。
The analysis of intact biological tissues by mass spectrometry (MS) has been pursued for more than three decades. However, mass spectrometric methods have always put strong constraints on the geometry and the preparation of these samples. Even with the recent advent of ambient ionization methods, not all of these restrictions have been lifted.[1–5]MS analysis of biomolecules in tissue has traditionally been achieved by desorption ionization methods including secondary ion mass spectrometry (SIMS),[6–11] matrix-assisted laser desorption (MALDI),[12–17, 19, 20] and desorption electrospray ionization (DESI)[4, 5, 18] methods. While desorption ionization methods are not appropriate for the analysis of vital (living) tissues, rapid thermal evaporation has the potential to establish the in situ, in vivo ionization of tissue constituents. The possible formation of organic ions from condensed-phase samples in a purely thermal process was initially proposed by Holland et al.,[21] and it was successfully demonstrated later.[22–24] The rationale of rapid heating was to achieve molecular evaporation rates comparable to the rate of decomposition, which results in the formation of a considerable quantity of gaseous molecules or molecular ions.