Exploring the capacity for anaerobic biodegradation of polycyclic aromatic hydrocarbons and naphthenic acids by microbes from oil-sands-process-affected waters
Exploring the capacity for anaerobic biodegradation of polycyclic aromatic hydrocarbons and naphthenic acids by microbes from oil-sands-process-affected waters
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DOI：
10.1016/j.ibiod.2014.12.016
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发表时间：
2016-03-01
影响因子：
4.8
通讯作者：
Whitby, Corinne
中科院分区：
文献类型：
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作者：
Folwell, Benjamin D.;McGenity, Terry J.;Whitby, Corinne
多环芳烃（PAHs）和环烷酸（NAs）都是化石燃料的天然组分，但它们也是广泛存在的有毒和环境持久性污染物。它们是油砂加工沃茨（OSPW）环境毒性的主要原因。本研究旨在研究多环芳烃芘和2-甲基萘，以及NAs金刚烷-1-羧酸和“天然”NA混合物（即，来自OSPW的酸可提取的NAs）在硫酸盐还原和产甲烷条件下通过来自油砂尾矿池的微生物群落进行。使用气相色谱-质谱法（GC MS），生物降解率进行了测量有关的细菌群落组成的变化。只有2-甲基萘显着降解后260天，与显着更多的降解硫酸盐还原（40%）比产甲烷条件下（25%）。在2-甲基萘的生物降解过程中，产生了一种主要的代谢产物，初步鉴定为2-萘甲酸。变性梯度凝胶电泳（DGGE）表明，在2-甲基萘的厌氧生物降解过程中，来自梭杆菌属，嗜碱菌属，脱硫杆菌属，Variovorax，Thaurea，噬氢菌属的物种的条带强度增加。尽管2-甲基萘的生物降解，这项研究表明，在厌氧条件下，NAs和高分子量的多环芳烃是主要的分子可能会坚持在OSPW。因此，需要采取替代补救战略。(C)2015由Elsevier Ltd.出版
Both polycyclic aromatic hydrocarbons (PAHs) and naphthenic acids (NAs) are natural components of fossil fuels, but they are also widespread toxic and environmentally persistent pollutants. They are the major cause of environmental toxicity in oil-sands-process waters (OSPW). This study aimed to investigate the anaerobic biodegradation of the PAHs pyrene and 2-methylnaphthalene, and the NAs adamantane-1-carboxylic acid and a "natural" NA mixture (i.e., acid-extractable NAs from OSPW) under sulfate-reducing and methanogenic conditions by a microbial community derived from an oil sands tailings pond. Using gas-chromatography mass spectrometry (GC MS), the rate of biodegradation was measured in relation to changes in bacterial community composition. Only 2-methylnaphthalene was significantly degraded after 260 days, with significantly more degradation under sulfate-reducing (40%) than methanogenic conditions (25%). During 2-methylnaphthalene biodegradation, a major metabolite was produced and tentatively identified as 2-naphthoic acid. Denaturing gradient gel electrophoresis (DGGE) demonstrated an increase in intensity of bands during the anaerobic biodegradation of 2-methylnaphalene, which derived from species of the genera Fusibacter, Alkaliphilus, Desulfobacterium, Variovorax, Thaurea, and Hydrogenophaga. Despite the biodegradation of 2-methylnaphthalene, this study demonstrates that, under anaerobic conditions, NAs and high-molecular-weight PAHs are the predominant molecules likely to persist in OSPW. Therefore alternative remediation strategies are required. (C) 2015 Published by Elsevier Ltd.