Transplantation of ocular stem cells: the role of injury in incorporation and differentiation of grafted cells in the retina
Transplantation of ocular stem cells: the role of injury in incorporation and differentiation of grafted cells in the retina
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DOI：
10.1016/s0042-6989(02)00688-0
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发表时间：
2003-04-01
期刊：
影响因子：
1.8
通讯作者：
Ahmad, I
中科院分区：
文献类型：
--
作者：
Chacko, DM;Das, AV;Ahmad, I
将移植细胞掺入宿主视网膜是成功的细胞替代疗法治疗视网膜变性的先决条件之一。为了检验损伤促进细胞掺入的假设，从视网膜、睫状上皮或角膜缘上皮分离干细胞/祖细胞，并移植到视网膜损伤大鼠的眼中。不同的干细胞/祖细胞群被整合到创伤或患病视网膜中，但不被整合到正常视网膜中。并入内层视网膜的细胞比例始终高于外层视网膜。移植的细胞表达对它们被并入的板层细胞特异的标记物，这表明特异性分化的线索位于内层和外层视网膜内。这些研究结果表明，损伤诱导的线索在促进眼干细胞/祖细胞的掺入中起着重要作用，而不管它们的来源或它们沿着特定视网膜亚系的分化。(C)2003爱思唯尔科技有限公司版权所有。
The incorporation of transplanted cells into the host retina is one of the prerequisites for successful cell replacement therapy to treat retinal degeneration. To test the hypothesis that injury promotes cell incorporation, stem cells/progenitors were isolated from the retina, ciliary epithelium or limbal epithelium and transplanted into the eyes of rats with retinal injury. Different stem cell/progenitor populations incorporated into traumatized or diseased retina but not into the normal retina. The proportion of cells incorporated into the inner retina was consistently higher than in the outer retina. The transplanted cells expressed markers specific to cells of the lamina into which they were incorporated suggesting that cues for specific differentiation are localized within tile inner and outer retina. These findings demonstrate that injury-induced cues play a significant role in promoting the incorporation of ocular stem cells/progenitors regardless of their origin or their differentiation along specific retinal sublineage. (C) 2003 Elsevier Science Ltd. All rights reserved.