Cardiac repair achieved by bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid patches in a rat of myocardial infarction model
Cardiac repair achieved by bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid patches in a rat of myocardial infarction model
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DOI：
10.1016/j.biomaterials.2012.04.030
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发表时间：
2012-08-01
期刊：
影响因子：
14
通讯作者：
Wang, Shoei-Shen
中科院分区：
文献类型：
--
作者：
Chi, Nai-Hsin;Yang, Ming-Chia;Wang, Shoei-Shen
将骨髓间充质干细胞/丝素蛋白/透明质酸（BMSC/SH）补片植入心肌梗死（MI）大鼠心脏，观察BMSC/SH补片对MI大鼠左室重构和心脏修复的影响。将45只大鼠分为4组：假手术组（Sham）、心肌梗死组（MI心脏，采用冷冻损伤技术诱导）。SH和BMSC/SH（分别为SH和BMSC/SH补片移植的MI心脏）。植入8周后，SH和BMSC/SH组的贴片完整且良好地粘附在MI区上，分别通过CD 68标记物检查没有和轻微的免疫反应，而MI组观察到严重的炎症。SH组显示了对MI区进行心脏修复的有效性。此外，BMSC/SH组显著改善左室壁厚度，通过超声心动图评估，并且具有高的递送BMSC活力，大大减少凋亡，显著促进新生血管形成，并刺激各种旁分泌因子如VEGF的分泌，通过实时PCR检测。与SH组和MI组相比，MI区中的总之，通过显示生物活性SH补片和基于BMSC的治疗的优点，证明了使用BMSC/SH补片修复MI心脏的治疗效果。(C)2012爱思唯尔有限公司保留所有权利。
Bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid (BMSC/SH) patches were implanted into myocardial infarction (MI) rat hearts to investigate the efficacies of them on enhancing left ventricular (LV) remodeling and cardiac repair. 45 rats were divided into four groups: Sham, MI (MI hearts, induced by a cryo-injury technique). SH and BMSC/SH (MI hearts with implantations of SH and BMSC/SH patches, respectively). After eight weeks of post-implantation, the patches for the SH and BMSC/SH groups were intact and well adhered on the MI zones with no and minor immunological responses, respectively, examined by a CD68 marker, while severe inflammation on the zones was observed for the MI group. The SH group showed the efficacy of cardiac repair on MI zones. Moreover, BMSC/SH group significantly improved the wall thickness of LV, assessed by echocardiography, and had high viability of delivery BMSC, largely reduced apoptosis, significantly promoted neo-vascularization and stimulated the secretions of various paracrine factors such as VEGF, examined by real-time PCR. in MI zones compared with those of the SH and MI groups. In conclusion, the therapeutic efficacies of using BMSC/SH patches for repairing MI hearts were demonstrated by showing the advantages of both bioactive SH patches and BMSC-based therapy. (C) 2012 Elsevier Ltd. All rights reserved.