In vivo penetration mechanics and mechanical properties of mouse brain tissue at micrometer scales.
In vivo penetration mechanics and mechanical properties of mouse brain tissue at micrometer scales.
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DOI：
10.1109/tbme.2008.2003261
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发表时间：
2009-01
期刊：
影响因子：
--
通讯作者：
Gall K
中科院分区：
文献类型：
--
作者：
Sharp AA;Ortega AM;Restrepo D;Curran-Everett D;Gall K
通过将各种装置植入大脑，对行为的神经元基础的理解和神经系统疾病的治疗取得了实质性进展。为了设计和优化下一代神经元植入物，同时努力减少组织损伤，有必要了解探针在相关长度尺度上的插入机制。不幸的是，在必要的微米尺度上，对大脑植入物相互作用的广泛理解在很大程度上是缺失的。本文对小鼠脑组织在体内的微米尺度穿透力学和材料特性进行了概括性描述。将圆柱形不锈钢探针插入小鼠大脑皮层和嗅球。在探针插入和取出过程中，连续测量探针大小、探针几何形状、插入速率、插入位置、动物年龄以及硬脑膜和硬脊膜的存在对结果力的影响。通过力学分析提取材料性能（模量、切削力和摩擦力）。使用刚性的、不可压缩的圆柱形探针，可以对探针设计和插入方法如何影响体内脑组织的渗透机制有一个大致的了解，这可以应用于大多数未来植入式装置的定量设计。
Substantial advancement in the understanding of the neuronal basis of behavior and the treatment of neurological disorders has been achieved via the implantation of various devices into the brain. To design and optimize the next generation of neuronal implants while striving to minimize tissue damage, it is necessary to understand the mechanics of probe insertion at relevant length scales. Unfortunately, a broad based understanding of brain-implant interactions at the necessary micrometer scales is largely missing. This paper presents a generalizable description of the micrometer scale penetration mechanics and material properties of mouse brain tissue in vivo. Cylindrical stainless steel probes were inserted into the cerebral cortex and olfactory bulb of mice. The effects of probe size, probe geometry, insertion rate, insertion location, animal age and the presence of the dura and pia on the resulting forces were measured continuously throughout probe insertion and removal. Material properties (modulus, cutting force, and frictional force) were extracted using mechanical analysis. The use of rigid, incompressible, cylindrical probes allows for a general understanding of how probe design and insertion methods influence the penetration mechanics of brain tissue in vivo that can be applied to the quantitative design of most future implantable devices.