Identification and quantification of ureaplasmas colonizing the respiratory tract and assessment of their role in the development of chronic lung disease in preterm infants
Identification and quantification of ureaplasmas colonizing the respiratory tract and assessment of their role in the development of chronic lung disease in preterm infants
复制标题
DOI：
10.1097/00006454-200109000-00006
复制
发表时间：
2001-09-01
影响因子：
3.6
通讯作者：
Robertson, JA
中科院分区：
文献类型：
--
作者：
Heggie, AD;Bar-Shain, D;Robertson, JA
背景。解脲支原体在早产儿慢性肺病（CLD）发展中的作用仍然存在争议。最近发现解脲支原体由两个物种组成，即解脲支原体和微小解脲支原体，这一发现在之前的 CLD 研究中并未考虑到。本研究探讨了这些新定义的物种的 CLD 发展与呼吸道定植之间的可能关系、它们在下呼吸道分泌物中的浓度以及肺表面活性物质治疗对出生体重 < 1500 g 的早产儿的这些关系的影响。方法。当医疗需要进行气道抽吸时，从插管婴儿身上收集气管内抽吸物（ETA）。 ETA保存在-80℃下，直到进行解脲支原体和人型支原体的定量培养。培养结果与 CLD 的发展相关。结果。在 2 年研究期间入院的 475 名婴儿（出生体重 < 1500 克）中，有 272 名因未插管或在获得预计到达时间之前拔管而被排除。另外 28 名婴儿在接受慢性肺病评估之前死亡、出院或转移。从剩下的 175 名婴儿中，有 66 名（38%）分离出解脲支原体。慢性肺病的发展与分离的解脲支原体种类或下呼吸道分泌物中解脲支原体的浓度之间没有发现统计学上显着的关联。这些结果并未因肺表面活性物质（Survanta）治疗而改变。结论。解脲支原体在下呼吸道定植似乎并不是早产儿慢性肺病的原因之一。
Background. The role of Ureaplasma urealyticum in the development of chronic lung disease (CLD) in preterm infants continues to be disputed. Recently U. urealyticum has been found to consist of two species, U. urealyticum and Ureaplasma parvum, a finding that has not been considered in previous studies of CLD. This study examined the possible relationships between development of CLD and respiratory colonization by these newly redefined species, their concentrations in lower respiratory secretions and the effect of pulmonary surfactant treatment on these relationships in preterm infants with birth weights < 1500 g.Methods. Endotracheal aspirates (ETA) were collected from intubated infants when airway suctioning was medically required. ETA were stored at -80 degreesC until quantitative cultures for ureaplasmas and Mycoplasma hominis were performed. Culture results were correlated with development of CLD.Results. Of 475 infants (birth weights < 1500 g) admitted during the 2-year study period, 272 were excluded because they were not intubated or were extubated before ETA could be obtained. An additional 28 infants died, were discharged or were transferred before they could be assessed for CLD. From the remaining 175 infants ureaplasmas were isolated from 66 (38%). No statistically significant associations were identified between development of CLD and the Ureaplasma species isolated, or concentration of ureaplasmas in lower respiratory secretions. These findings were not altered by treatment with pulmonary surfactant (Survanta).Conclusion. Lower respiratory colonization by ureaplasmas does not appear to be a contributory cause of CLD in preterm infants.