Transcriptomic analysis reveals that non-forage or forage fiber source promotes rumen development through different metabolic processes in lambs
Transcriptomic analysis reveals that non-forage or forage fiber source promotes rumen development through different metabolic processes in lambs
复制标题
转录组分析表明，非饲料或饲料纤维来源通过不同的代谢过程促进羔羊瘤胃发育
DOI：
10.1080/10495398.2021.2011738
复制
发表时间：
2021-12-07
影响因子：
3.7
通讯作者：
Weng, Xiuxiu
中科院分区：
文献类型：
--
作者：
Liu, Ting;Li, Fadi;Weng, Xiuxiu
Abstract Dietary fiber supplementation can stimulate rumen development in lambs during the pre-weaning period. However, it is unclear whether different sources of fiber have varying effects on rumen development. This study aimed to investigate the molecular mechanism of rumen morphological and functional development based on non-forage or forage as a starter dietary fiber source. Twenty-four male Hu lambs with similar body weights (BW, 3.67 ± 0.08 kg) were selected and divided into two groups that received diets supplemented with either alfalfa hay (AH) or soybean hull (SH). At the age of 70 days, six lambs were slaughtered from each treatment group for rumen fermentation and morphological analyses. Three samples of the rumen tissue from the ventral sac were collected for transcriptomic analysis. The results identified 633 differentially expressed genes (DEGs), of which 210 were upregulated and 423 were downregulated in the SH group compared with those in the AH group. The upregulated DEGs were most enriched in the immune function and proteolysis pathways, whereas the downregulated DEGs were mainly involved in cell proliferation, apoptosis, and differentiation pathways. These findings indicated that non-forage as a starter dietary fiber source improved immune function and enhanced nitrogen utilization, whereas forage facilitated rumen morphological development.
