Rare-Cell Enrichment by a Rapid, Label-Free, Ultrasonic Isopycnic Technique for Medical Diagnostics
Rare-Cell Enrichment by a Rapid, Label-Free, Ultrasonic Isopycnic Technique for Medical Diagnostics
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通过快速、无标记、超声波等密度技术进行医学诊断的稀有细胞富集
DOI：
10.1002/ange.201310401
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发表时间：
2014
影响因子：
--
通讯作者：
Bourquin Y
中科院分区：
文献类型：
--
作者：
Bourquin Y
One significant challenge in medical diagnostics lies in the development of label‐free methods to separate different cells within complex biological samples. Here we demonstrate a generic, low‐power ultrasonic separation technique, able to enrich different cell types based upon their physical properties. For malaria, we differentiate between infected and non‐infected red blood cells in a fingerprick‐sized drop of blood. We are able to achieve an enrichment of circulating cells infected by the ring stage of the parasite over nonparasitized red blood cells by between two and three orders of magnitude in less than 3 seconds (enabling detection at parasitemia levels as low as 0.0005 %). In a second example, we also show that our methods can be used to enrich different cell types, concentratingTrypanosomain blood at very low levels of infection, on disposable, low‐cost chips.
