An insight into statistical refractive index properties of cell internal structure via low-coherence statistical amplitude microscopy
An insight into statistical refractive index properties of cell internal structure via low-coherence statistical amplitude microscopy
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DOI：
10.1364/oe.18.021950
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发表时间：
2010-10-11
期刊：
影响因子：
3.8
通讯作者：
Liu, Yang
中科院分区：
文献类型：
--
作者：
Wang, Pin;Bista, Rajan K.;Liu, Yang
折射率特性，特别是在纳米尺度下，在癌症诊断和筛查中显示出巨大的潜力。由于生物细胞本身的复杂性和微弱的折射率起伏，其内部结构的重建一直是一个挑战。在本文中，我们提出了一个简单而实用的方法来获得生物细胞内的内部折射率起伏的统计特性与一种新的光学显微镜方法-低相干统计振幅显微镜（SAM）。我们验证了SAM的能力来表征细胞内部结构的统计特性，这是由一维高斯随机场的数值模型描述。我们证明了SAM在癌症检测中的潜力与肠道癌变的动物模型-多发性肠肿瘤小鼠模型。我们发现，SAM衍生的细胞核结构的统计特性可以检测到微妙的变化，否则无法检测到的常规细胞病理学。(C)2010年美国光学学会
Refractive index properties, especially at the nanoscale, have shown great potential in cancer diagnosis and screening. Due to the intrinsic complexity and weak refractive index fluctuation, the reconstruction of internal structures of a biological cell has been challenging. In this paper, we propose a simple and practical approach to derive the statistical properties of internal refractive index fluctuations within a biological cell with a new optical microscopy method - Low-coherence Statistical Amplitude Microscopy (SAM). We validated the capability of SAM to characterize the statistical properties of cell internal structures, which is described by numerical models of one-dimensional Gaussian random field. We demonstrated the potential of SAM in cancer detection with an animal model of intestinal carcinogenesis - multiple intestinal neoplasia mouse model. We showed that SAM-derived statistical properties of cell nuclear structures could detect the subtle changes that are otherwise undetectable by conventional cytopathology. (C) 2010 Optical Society of America