Expression and function of DNAM-1 on human B-lineage cells
Expression and function of DNAM-1 on human B-lineage cells
复制标题
DOI：
10.1002/cyto.b.21859
复制
发表时间：
2019-11-29
影响因子：
3.4
通讯作者：
Shibuya, Kazuko
中科院分区：
文献类型：
--
作者：
Nagayama-Hasegawa, Yuko;Honda, Shin-ichiro;Shibuya, Kazuko
背景 尽管 DNAM-1 是一种在人类大多数 NK 细胞、CD8(+) T 细胞、CD4(+) T 细胞、单核细胞和血小板上组成型表达的激活受体，但一些证据表明 B 系细胞中的一小部分也表达 DNAM-1。然而，DNAM-1 在 B 系细胞上的表达谱及其功能仍然不清楚。此前的报道显示，外周血中相当数量的白细胞（包括B细胞）与血小板结合。因此，之前报告中通过流式细胞术分析确定的DNAM-1(+) B谱系细胞的比例可能被高估。方法我们检测血小板是否与B细胞结合，然后根据排除血小板结合的B细胞后的成熟阶段，分析B系细胞亚群上DNAM-1的表达。我们还评估了 DNAM-1 在 IL-10 中的参与以及用 CpG-ODN 刺激的培养 B 谱系细胞的抗体产生。结果外周血中大约10%的人DNAM-1(+) CD19(+) B细胞与血小板结合，导致DNAM-1(+) B细胞比例过高。排除血小板结合 B 细胞后，我们发现在记忆 B 细胞、浆母细胞和浆细胞亚群中检测到 DNAM-1 表达，并通过 CpG-ODN 刺激上调。此外，DNAM-1 参与 CpG-ODN 刺激后 B 细胞产生 IL-10 和抗体。结论 DNAM-1可能参与B系细胞介导的免疫反应。
Background Although DNAM-1 is an activating receptor constitutively expressed on the majority of NK cells, CD8(+) T cells, CD4(+) T cells, monocytes, and platelets in human, several evidences demonstrated that a small population in B-lineage cells also expressed DNAM-1. However, the expression profile of DNAM-1 on B-lineage cells and its function remain obscure. Previous reports revealed that a considerable number of leukocytes including B cells in the peripheral blood conjugated to platelet. Thus, the proportion of DNAM-1(+) B-lineage cells determined by flow cytometry analysis in the previous reports might be overestimated. Methods We examined whether platelets conjugate B cells and then analyzed the expression of DNAM-1 on the subpopulations of B-lineage cells according to their maturation stages after exclusion of platelet-conjugated B cells. We also assessed the involvement of DNAM-1 in IL-10 and antibody production from cultured B-lineage cells stimulated with CpG-ODN. Results Approximately 10% of human DNAM-1(+) CD19(+) B cells in the peripheral blood conjugated to platelets, resulting in the overestimation of the proportion of DNAM-1(+) B cells. After exclusion of platelet-conjugating B cells, we show that DNAM-1 expression was detected on subpopulations of memory B cells, plasmablasts, and plasma cells and upregulated by stimulation with CpG-ODN. Moreover, DNAM-1 was involved in IL-10 and antibody productions by B cells after CpG-ODN stimulation. Conclusions DNAM-1 may be involved in B-lineage cell-mediated immune responses.