Transcriptome Analyses of Diaphorina citri Midgut Responses to Candidatus Liberibacter Asiaticus Infection
Transcriptome Analyses of Diaphorina citri Midgut Responses to Candidatus Liberibacter Asiaticus Infection
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DOI：
10.3390/insects11030171
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发表时间：
2020-03-01
期刊：
影响因子：
3
通讯作者：
Lu, Zhan-Jun
中科院分区：
文献类型：
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作者：
Yu, Hai-Zhong;Li, Ning-Yan;Lu, Zhan-Jun
亚洲柑橘木虱 (ACP)、Diaphorina citri Kuwayama（半翅目：Liviidae）是柑橘绿化病 Candidatus Liberibacter asiaticus (CLas) 的重要传播媒介。 D. citri 中肠表现出对抗 CLas 感染的重要组织屏障。然而，中肠对 CLas 感染反应的分子机制尚未全面阐明。在这项研究中，我们通过比较转录组分析，鉴定了 CLas 感染后中肠中的 778 个差异表达基因（DEG），其中包括 499 个上调的 DEG 和 279 个下调的 DEG。功能注释分析表明，这些差异表达基因与泛素化、免疫反应、核糖体、内吞作用、细胞骨架和杀虫剂抗性相关。 KEGG富集分析显示，大多数DEG主要参与内吞作用和核糖体。通过逆转录定量PCR（RT-qPCR）进一步验证了总共14个DEG功能。这项研究将有助于我们了解 CLas 和 D. citri 之间的分子相互作用。
The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), is an important transmission vector of the citrus greening disease Candidatus Liberibacter asiaticus (CLas). The D. citri midgut exhibits an important tissue barrier against CLas infection. However, the molecular mechanism of the midgut response to CLas infection has not been comprehensively elucidated. In this study, we identified 778 differentially expressed genes (DEGs) in the midgut upon CLas infection, by comparative transcriptome analyses, including 499 upregulated DEGs and 279 downregulated DEGs. Functional annotation analysis showed that these DEGs were associated with ubiquitination, the immune response, the ribosome, endocytosis, the cytoskeleton and insecticide resistance. KEGG enrichment analysis revealed that most of the DEGs were primarily involved in endocytosis and the ribosome. A total of fourteen DEG functions were further validated by reverse transcription quantitative PCR (RT-qPCR). This study will contribute to our understanding of the molecular interaction between CLas and D. citri.