Improved Maule color reaction provides more detailed information on syringyl lignin distribution in hardwood
Improved Maule color reaction provides more detailed information on syringyl lignin distribution in hardwood
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改进的 Maule 颜色反应提供了有关硬木中紫丁香基木质素分布的更详细信息
DOI：
10.1007/s10086-016-1536-9
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发表时间：
2016
影响因子：
2.9
通讯作者：
Keiji Takabe
中科院分区：
文献类型：
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作者：
Daichi Yamashita;Satoshi Kimura;Masahisa Wada;Keiji Takabe
采用三羟甲基氨基甲烷-盐酸（Tris-HCl）缓冲液与荧光显微镜联用，改进了传统的Mäule显色反应。与传统处理相比，新处理的Mäule处理的硬木的紫红色更稳定。此外，Mäule处理的木材样品在蓝光（460-495 nm）激发下具有特征荧光。对白色白桦（Betula platyphylla）的研究表明，富含异戊烯基和愈创木脂基的细胞壁分别发出红色和黄色荧光。用新的Mäule试剂处理后，测量硬木和软木粉末的荧光光谱，结果表明，含有愈创木基木质素的硬木粉末发出红色荧光，而含有愈创木基木质素的软木粉末在蓝光激发下发出绿色荧光。与传统的Mäule比色法相比，该方法具有许多优点。
The traditional Mäule color reaction was improved by use of tris(hydroxymethyl) aminomethane-hydrochloride acid (Tris–HCl) buffer coupled with a fluorescence microscope. The purple-red color of Mäule-treated hardwood was more stable with the novel treatment than with traditional treatment. In addition, Mäule-treated wood samples had a characteristic fluorescence when excited with blue (460–495 nm) light. Examination of white birch (Betula platyphylla) showed that syringyl-rich and guaiacyl-rich cell walls emitted red and yellow fluorescence, respectively. Measuring the fluorescence spectra of hardwood and softwood powders after treatment with the new Mäule reagents showed that hardwood powder containing syringyl lignin emitted a red fluorescence, whereas softwood powder containing guaiacyl lignin emitted a green fluorescence when excited with blue light. In conclusion, this improved technique has many advantages compared with the traditional Mäule color test.