Impact of tuberculosis (TB) on HIV-1 activity in dually infected patients
Impact of tuberculosis (TB) on HIV-1 activity in dually infected patients
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DOI：
10.1046/j.1365-2249.2001.01401.x
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发表时间：
2001-02-01
影响因子：
4.6
通讯作者：
Whalen, CW
中科院分区：
文献类型：
--
作者：
Toossi, Z;Mayanja-Kizza, H;Whalen, CW
hiv -1感染者的活动性结核与hiv -1相关免疫缺陷和死亡率增加有关。我们评估了HIV-1感染肺结核患者（HIV/TB）和非结核症状HIV-1感染患者（HIV）的血浆病毒载量。HIV-1在HIV/TB中的载量高于CD4计数较高的HIV (500,500 /mul) (P < 0.01)，而CD4计数较低的HIV （500,500 /mul）无显著差异。我们还通过逆转录聚合酶链反应评估了HIV/TB和CD4匹配的健康HIV感染患者（HIV/C）外周血单个核细胞（PBMC）和血清中HIV-1基因表达的状态，其范围为CD4 （bbb900 /mul至< 200/mul）。血清中HIV-1 RNA和PBMC中HIV-1 RNA相互相关，并且HIV/TB中HIV-1 RNA和PBMC中HIV-1 RNA明显高于CD4计数较高的HIV/C。此外，在hiv -1感染患者抗结核化学预防的纵向研究中，10名患结核病的受试者在诊断结核病之前、当时和之后都有血清学检查。这些HIV/TB患者在诊断为TB时病毒载量增加（平均2.5倍）（P < 0.05）。总的来说，这些数据表明HIV-1的转录活性在HIV-1感染的活动性结核病患者中增强，特别是在早期HIV-1疾病期间。由于结核病通常是一种早期HIV-1机会性感染，它可能特别有利于早期病毒复制和传播，因此有助于HIV-1疾病的进展。
Active TB in HIV-1-infected subjects is associated with increased HIV-1-related immunodeficiency and mortality. We assessed plasma viral load in HIV-1-infected patients with pulmonary TB (HIV/TB) and non-TB symptomatic HIV-1-infected patients (HIV). HIV-1 load was higher in HIV/TB compared with HIV at higher CD4 counts (> 500/mul) (P < 0.01), but not at lower CD4 counts (< 500/mul). We also evaluated the status of HIV-1 gene expression in peripheral blood mononuclear cells (PBMC) and serum from HIV/TB and CD4-matched healthy HIV-infected patients (HIV/C) by reverse transcriptase-polymerase chain reaction over a range of CD4 (> 900/mul to < 200/mul). HIV-1 RNA in serum and PBMC correlated to one another, and both were markedly higher in HIV/TB compared with HIV/C with higher CD4 counts. Also, during a longitudinal study of anti-tuberculous chemoprophylaxis in HIV-1-infected patients, 10 subjects who developed TB had serologies before, at the time, and after the diagnosis of TB. These HIV/TB patients had an increase in viral load (average 2.5-fold) at the time of diagnosis of TB (P < 0.05). Overall, these data indicate that the transcriptional activity of HIV-1 is enhanced in HIV-1-infected patients with active TB, especially during early HIV-1 disease. As TB often is an early HIV-1 opportunistic infection, it may particularly favour early viral replication and dissemination, and therefore contribute to progression of HIV-1 disease.