Expression, Localization, and Protein Interactions of the Partitioning Proteins in the Gonococcal Type IV Secretion System.
Expression, Localization, and Protein Interactions of the Partitioning Proteins in the Gonococcal Type IV Secretion System.
复制标题
DOI：
10.3389/fmicb.2021.784483
复制
发表时间：
2021
影响因子：
5.2
通讯作者：
Dillard JP
中科院分区：
文献类型：
--
作者：
Callaghan MM;Koch B;Hackett KT;Klimowicz AK;Schaub RE;Krasnogor N;Dillard JP
分配蛋白作为分裂过程中染色体和质粒分离的分子组织者已得到充分研究，但人们对分配蛋白在 IV 型分泌系统中发挥的作用知之甚少。分泌单链 DNA (ssDNA) 的淋球菌 T4SS 有两种分配蛋白：ParA 和 ParB。这些蛋白质与松弛酶 TraI 协同作用，作为 IV 型分泌的重要促进剂。细菌双杂交实验确定了每种分配蛋白和松弛酶之间的相互作用。亚细胞分级分离表明，ParA 存在于细胞膜中，而 ParB 主要存在于细胞膜中，但部分蛋白质存在于可溶性部分中。由于已知 TraI 与膜相关，因此这些数据表明淋球菌松弛体是一种膜相关复合物。此外，我们发现ParA和ParB的翻译是由RNA开关控制的。茎环序列内的不同突变预计会改变这种 RNA 结构的折叠，从而大大增加或减少分配蛋白的水平。
Partitioning proteins are well studied as molecular organizers of chromosome and plasmid segregation during division, however little is known about the roles partitioning proteins can play within type IV secretion systems. The single-stranded DNA (ssDNA)-secreting gonococcal T4SS has two partitioning proteins, ParA and ParB. These proteins work in collaboration with the relaxase TraI as essential facilitators of type IV secretion. Bacterial two-hybrid experiments identified interactions between each partitioning protein and the relaxase. Subcellular fractionation demonstrated that ParA is found in the cellular membrane, whereas ParB is primarily in the membrane, but some of the protein is in the soluble fraction. Since TraI is known to be membrane-associated, these data suggest that the gonococcal relaxosome is a membrane-associated complex. In addition, we found that translation of ParA and ParB is controlled by an RNA switch. Different mutations within the stem-loop sequence predicted to alter folding of this RNA structure greatly increased or decreased levels of the partitioning proteins.
登录
查看更多内容
DOI：
10.1099/mic.0.081216-0
发表时间：
2014-11
期刊：
Microbiology (Reading, England)
影响因子：
--
作者：
Bartosik AA;Glabski K;Jecz P;Lasocki K;Mikosa M;Plochocka D;Thomas CM;Jagura-Burdzy G
通讯作者：
Jagura-Burdzy G
影响因子：
3.2
作者：
Kohler, Petra L.;Chan, Yolande A.;Dillard, Joseph P.
通讯作者：
Dillard, Joseph P.
影响因子：
3.6
作者：
Hamilton, HL;Domínguez, NM;Dillard, JP
通讯作者：
Dillard, JP
影响因子：
3.2
作者：
Hamilton, HL;Schwartz, KJ;Dillard, JP
通讯作者：
Dillard, JP
影响因子：
64.8
作者：
Hemmi, H;Takeuchi, O;Akira, S
通讯作者：
Akira, S