The utility of the hematoxylin and eosin staining in patients with suspected Hirschsprung disease
The utility of the hematoxylin and eosin staining in patients with suspected Hirschsprung disease
复制标题
DOI：
10.1186/s12893-017-0267-1
复制
发表时间：
2017-06-19
期刊：
影响因子：
1.9
通讯作者：
Gunadi
中科院分区：
文献类型：
--
作者：
Setiadi, Josephine Amanda;Dwihantoro, Andi;Gunadi
背景：虽然免疫组织化学（IHC）方法在发达国家已广泛用于诊断先天性巨结肠（HSCR），但在发展中国家，其使用的研究很少，苏木精和伊红（HE）染色是诊断 HSCR 的关键要素。我们旨在以印度尼西亚 S100 IHC 作为参考标准，确定 HE 染色在 HSCR 诊断中的准确性。方法：对 2013 年 1 月至 2015 年 8 月在印度尼西亚日惹 Sardjito 医院进行的所有疑似 HSCR 患者进行的组织病理学检查进行回顾性分析。结果：我们的研究包括 23 名 HSCR 患者：9 名男性和 14 名女性。 HE染色显示14例阴性（缺乏神经节细胞）病例（61％）和9例阳性（存在神经节细胞）病例（39％）。在S100 IHC中，HE染色阳性的9例中，6例（67%）被确认有神经节细胞；在 HE 染色呈阴性的 14 例中，S100 IHC 染色呈阴性，12 例（86%）呈阴性，2 例（14%）呈阳性。 HE染色的敏感性、特异性、阳性预测值、阴性预测值和准确率分别为80%（95% CI：0.51-0.95）、75%（95% CI：0.36-0.96）、85.7%（95% CI：0.56-0.98）、66.7%（95% CI：0.31-0.91）和分别为 78.3% (95% CI: 0.58-0.90)。结论：我们的研究表明 HE 染色对于 HSCR 的诊断具有相对中等的准确性。鉴于发展中国家更昂贵的 IHC 技术的资源分配有限，仍然建议使用 HE 染色来诊断 HSCR。
Background: While immunohistochemistry (IHC) methods have been widely conducted for the diagnosis of Hirschsprung disease (HSCR) in developed countries, there are very few studies on their use in developing countries where hematoxylin and eosin (HE) staining is a key element of the diagnosis of HSCR. We aimed to determine the accuracy of HE staining in the diagnosis of HSCR using S100 IHC as the reference standard in Indonesia.Methods: All histopathology performed for the suspicion of HSCR patients from January 2013 to August 2015 in Dr. Sardjito Hospital, Yogyakarta, Indonesia, were retrospectively reviewed.Results: Our study included 23 HSCR patients: 9 males and 14 females. The HE staining revealed 14 negative (absence of ganglion cells) cases (61%) and 9 positive (presence of ganglion cells) cases (39%). In S100 IHC, out of the 9 positive cases by HE staining, 6 (67%) were confirmed for having ganglion cells; and out of the 14 negative cases by HE staining, 12 (86%) were reported negative and 2 (14%) were positive by S100 IHC staining. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy rates of the HE staining were 80% (95% CI: 0.51-0.95), 75% (95% CI: 0.36-0.96), 85.7% (95% CI: 0.56-0.98), 66.7% (95% CI: 0.31-0.91), and 78.3% (95% CI: 0.58-0.90), respectively.Conclusions: Our study showed that HE staining has relatively moderate accuracy for the diagnosis of HSCR. The use of HE staining is still recommended for the diagnosis of HSCR given the limitation of resource allocation for more expensive IHC technologies in developing countries.