Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa
Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa
复制标题
秋水仙碱不影响毛白杨诱导2n花粉的活力
DOI：
10.14214/sf.10132
复制
发表时间：
2019
期刊：
影响因子：
1.8
通讯作者：
Zhang Pingdong
中科院分区：
文献类型：
--
作者：
Liu Yan;Zhang Yuan;Zhou Qing;Wu Jian;Zhang Pingdong
秋水仙碱被广泛用作诱变剂，诱导植物产生二倍体配子。然而，秋水仙碱是否影响诱导花粉的活力尚不清楚。为了明确秋水仙碱是否影响诱导花粉的活力，我们在Carrière中用秋水仙碱诱导产生二倍体花粉，然后将花粉萌发并与正常配子杂交产生三倍体。结果表明，主要减数分裂时期和秋水仙碱注射次数对诱导的2n花粉发生率有显著影响。早衰时给予秋水仙碱注射，每芽花粉产量显著降低（<e:1>, <, <e:1>, 0.001）。秋水仙碱诱导的2n花粉外质结构与天然2n花粉形态相似。花粉萌发试验表明，诱导的二倍体花粉与天然2n花粉的萌发率也无显著差异，通过秋水仙碱诱导的花粉杂交可产生68个三倍体。结果表明，秋水仙碱能诱导产生2n花粉，不会导致诱导的二倍体花粉功能障碍。离体毛杨；tomentosa
Colchicine is widely used as a mutagen to induce production of diploid gametes in plants. However, whether colchicine affects induced pollen viability remains unclear. To clarify whether colchicine affected the viability of induced pollen, we induced production of diploid pollen by colchicine, followed by pollen germination and crossing induced pollen with normal gametes to produce triploid in Carrière. The results showed that the predominant meiotic stages and the number of colchicine injections had significant effects on the occurrence rates of induced 2n pollen. When the colchicine injection was given at diakinesis, a significant decrease in the pollen production per bud was observed (â<â0.001). The morphology of the colchicine-induced 2n pollen was similar to that of the natural 2n pollen in its ectexine structure. The pollen germination experiments revealed that there was also no significant difference in germination rates between the induced diploid pollen and natural 2n pollen grains, and 68 triploids were created by crossing colchicine-induced pollen. Our findings revealed that colchicine injection could induce to produce 2n pollen and will not lead to dysfunction of induced diploid pollen.in vitroPopulus tomentosapP. tomentosa