Different expression of α and β mitochondrial estrogen receptors in the aging rat brain: Interaction with respiratory complex V
Different expression of α and β mitochondrial estrogen receptors in the aging rat brain: Interaction with respiratory complex V
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DOI：
10.1016/j.exger.2010.01.015
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发表时间：
2010-07-01
影响因子：
3.9
通讯作者：
Cerbon, Marco
中科院分区：
文献类型：
--
作者：
Alvarez-Delgado, Carolina;Adriana Mendoza-Rodriguez, C.;Cerbon, Marco
最近的证据表明，激素对线粒体的影响可能是由线粒体雌激素受体（mtER）介导的。这些受体是在不同生理条件下对线粒体有益的雌激素效应的新候选者。本研究的目的是研究脑老化过程中mtER的表达。我们用Western blot分析了年轻成年（3个月）和老年（18个月）雌性Wistar大鼠皮质、海马和下丘脑线粒体中mtER α和mtER β的表达。此外，我们还通过免疫共沉淀试验探讨了线粒体ER β与呼吸复合物V的相互作用。结果表明，mtER α和mtER β存在于年轻和老年脑线粒体中。我们还表明，线粒体ERs表达为变体，并具有大脑区域特异性分布。检测到的主要mtER变异体是61和55 KDa的mtER α和63和52 KDa的mtER β。然而，我们没有观察到研究的两个年龄组之间的mtER α或β含量的差异。此外，我们发现，线粒体ER β与复合物V相互作用。总体结果表明，在不同的大脑区域中，线粒体ER α和线粒体ER β变体的表达存在差异，这表明它们可能参与衰老过程中大脑的不同功能。(C)2010年爱思唯尔公司All rights reserved.
Recent evidence suggests that hormonal effects on mitochondria could be mediated by mitochondrial estrogen receptors (mtERs). These receptors are new candidates for the beneficial estrogenic effects on mitochondria in different physiological conditions. The aim of this investigation was to study mtER expression during brain aging. We analyzed mtER alpha and mtER beta expression in cortical, hippocampal and hypothalamic mitochondria of young adult (3 months) and aged (18 months) female Wistar rats by Western blot. In addition, we explored the interaction of mtER beta with respiratory complex V by using coimmunoprecipitation assays. The results show that mtER alpha and mtER beta are present in young and aged brain mitochondria. We also demonstrate that mtERs are expressed as variants and have a brain region specific distribution. The predominant mtER variants detected were of 61 and 55 KDa for mtER alpha and of 63 and 52 KDa for mtER beta. However, we did not observe differences in the mtER alpha or beta content between the two age groups studied. Additionally, we show that mtER beta interacts with complex V.The overall results demonstrate that there is a differential expression of mtER alpha and mtER beta variants in different brain areas, indicating that they may participate in different functions in the brain during aging. (C) 2010 Elsevier Inc. All rights reserved.