Disruption of egg‐specific protein causes female sterility in Bombyx mori
Disruption of egg‐specific protein causes female sterility in Bombyx mori
复制标题
卵特异性蛋白的破坏导致家蚕雌性不育
DOI：
10.1111/1744-7917.12904
复制
发表时间：
2021-02
期刊：
影响因子：
4
通讯作者：
Xia Xu;Yaohui Wang;Zulian Liu;Yong-qiang Wang;Lin He;Kai Li;Yongping Huang
中科院分区：
文献类型：
--
作者：
Xia Xu;Yaohui Wang;Zulian Liu;Yong-qiang Wang;Lin He;Kai Li;Yongping Huang
卵黄蛋白是卵生动物胚胎和早期幼虫发育的主要营养来源。因此，卵黄发生对生殖至关重要。家蚕是一种模式鳞翅目昆虫，其中有三种卵黄蛋白：卵黄蛋白，30 kDa蛋白和卵特异性蛋白（Esp）。在这项研究中，我们通过转基因成簇规则间隔回文重复序列（CRISPR）/ CRISPR相关蛋白9技术介导的家蚕突变来探索Esp的基因功能。我们发现Esp突变导致雌性不育，但对雄性育性没有影响。雌性突变体在交配后可以产卵，但卵比野生型雌性所产的卵更小，颜色更浅。最重要的发现是，雌性突变体产下的卵不能孵化。此外，我们观察到Esp突变引起的雌性不育通过连续世代的稳定遗传。因此，Esp编码的卵黄蛋白是女性生殖成功的关键，是一个潜在的害虫防治目标。
Yolk proteins are the main source of nutrients during embryonic and early larval development in oviparous animals. Therefore, vitellogenesis is crucial for reproduction. The silkworm, Bombyx mori, is a model lepidopteran insect in which there are three yolk proteins: vitellin, 30‐kDa protein, and egg‐specific protein (Esp). In this study, we explored the gene function of Esp through transgenic clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR‐associated protein 9 technology‐mediated mutations in the silkworm. We found that Esp mutation resulted in female sterility but had no effect on male fertility. Female mutants could lay eggs after mating, but the eggs were smaller and lighter colored than those laid by wild‐type females. The most important finding is that the eggs laid by female mutants did not hatch. Furthermore, we observed stable inheritance of female sterility caused by Esp mutation through successive generations. Thus, Esp encodes a yolk protein that is crucial for female reproductive success and is a potential target for pest control.