Hydrolysis of animal manure lignocellulosics for reducing sugar production.
Hydrolysis of animal manure lignocellulosics for reducing sugar production.
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DOI：
10.1016/s0960-8524(03)00166-4
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发表时间：
2004
影响因子：
11.4
通讯作者：
Z. Wen;W. Liao;Shulin Chen
中科院分区：
文献类型：
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作者：
Z. Wen;W. Liao;Shulin Chen
将动物粪便转化为增值产品为此类材料的处理和处置提供了潜在的替代方案。木质纤维素是动物粪便的主要成分，是一种未开发的生物资源。在这项工作中，开发了一种用于将粪肥木质纤维素水解成可发酵糖的方法。当用3%硫酸在110 °C下预处理1 h时，半纤维素被完全降解为主要的阿拉伯糖、半乳糖和木糖。然后用纤维素分解酶Celluclast-1.5L和Novozyme-188处理预处理的材料以水解纤维素。确定了最佳酶负荷为13 FPU纤维素酶/g底物和5 IU β-葡萄糖苷酶/g底物。最佳温度和pH分别确定为46 °C和4.8。底物浓度为50 g/l有利于葡萄糖浓度（水解产物）和葡萄糖产量（基于每100 g粪肥）。还发现，590 μm的减小的粒度导致高葡萄糖产率，并且粒度的进一步减小不会增加产率。对于所研究的每种粒度，添加2%吐温-80导致葡萄糖产率至少提高20%。优化的水解工艺实现了11.32 g/100 g粪肥的葡萄糖产率，相当于约40%的纤维素转化率。
Converting animal manure into value-added products provides a potential alternative for treatment and disposal of such materials. Lignocellulosics are a major component of animal manure and represent an undeveloped bioresource. In this work, a process was developed for hydrolyzing manure lignocellulosics into fermentable sugars. When raw dairy manure was pre-treated with 3% sulfuric acid at 110 °C for 1 h, hemicellulose was completely degraded into mainly arabinose, galactose and xylose. The pretreated materials were then treated with cellulolytic enzymes, Celluclast-1.5L and Novozyme-188, to hydrolyze the cellulose. The optimal enzyme loadings were identified as 13 FPU cellulase/g substrate and 5 IU β-glucosidase/g substrate. The optimal temperature and pH were determined to be 46 °C and 4.8, respectively. A substrate concentration of 50 g/l favored both glucose concentration (in hydrolysate) and glucose yield (based on per 100 g manure). It was also found that a reduced particle size of 590-μm resulted in a high glucose yield with further decreases in particle size not increasing the yield. For each particle size investigated, the addition of 2% tween-80 resulted in at least 20% improvement in glucose yield. The optimized hydrolysis process achieved a glucose yield of 11.32 g/100 g manure, which corresponded to about 40% cellulose conversion.