Cloning and Heterologous Expression of the Thioviridamide Biosynthesis Gene Cluster from Streptomyces olivoviridis
Cloning and Heterologous Expression of the Thioviridamide Biosynthesis Gene Cluster from Streptomyces olivoviridis
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DOI：
10.1128/aem.01978-13
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发表时间：
2013-11-01
影响因子：
4.4
通讯作者：
Hayakawa, Yoichi
中科院分区：
文献类型：
--
作者：
Izawa, Masumi;Kawasaki, Takashi;Hayakawa, Yoichi
硫代维胺是橄榄绿链霉菌（Streptomycesolivoviridis）中一种独特的含有五个硫代酰胺键的肽类抗生素。基因组测序草图揭示了编码硫代病毒胺前体肽的基因（tvaA基因）。通过在变铅青链霉菌中异源生产硫代维瑞胺，鉴定了硫代维瑞胺生物合成基因簇。
Thioviridamide is a unique peptide antibiotic containing five thioamide bonds from Streptomyces olivoviridis. Draft genome sequencing revealed a gene (the tvaA gene) encoding the thioviridamide precursor peptide. The thioviridamide biosynthesis gene cluster was identified by heterologous production of thioviridamide in Streptomyces lividans.