Quantitative analysis of human serum albumin-drug interactions using reversed-phase and ion-exchange liquid chromatography
Quantitative analysis of human serum albumin-drug interactions using reversed-phase and ion-exchange liquid chromatography
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使用反相和离子交换液相色谱定量分析人血清白蛋白-药物相互作用
DOI：
10.1016/s0003-2670(98)00605-9
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发表时间：
1999
影响因子：
6.2
通讯作者：
T. Kinoshita
中科院分区：
文献类型：
--
作者：
T. Hanai;R. Miyazaki;T. Kinoshita
建立了一种间接测定蛋白质-药物结合亲和力的新的高效液相色谱系统，并以纯化的人血清白蛋白为原料，用改良Hummer-Dryer法测定了酸性药物的lognK值。用反相法和离子交换液色谱法测定了酸性药物的保留因子。键合相为丁基、苯丙基和胍基三种相，在pH为7.5的磷酸钠缓冲溶液中测定了它们的保留因子。组合保留因子与测得的lognK值相关，相关系数为0.948。
A new liquid chromatographic system was developed to measure protein-drug binding affinity indirectly and evaluated using lognK values of acidic drugs measured by a modified Hummer–Dryer method using purified human serum albumin. The retention factors of acidic drugs were measured in two reversed-phases and an ion-exchange liquid chromatography. The bonded phases were butyl, phenylpropyl and guanidino phases, and their retention factors were measured in sodium phosphate buffer of pH7.5. The combined retention factors were correlated with the measured lognK values; the correlation coefficient was 0.948.