Induction of Human Regulatory T Cells with Bacterial Superantigens
Induction of Human Regulatory T Cells with Bacterial Superantigens
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DOI：
10.1007/978-1-4939-3344-0_16
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发表时间：
2016-01-01
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影响因子：
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通讯作者：
Llewelyn, Martin J.
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文献类型：
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作者：
Caserta, Stefano;Taylor, Amanda L.;Llewelyn, Martin J.
抑制免疫效应细胞活化的调节性T细胞（Tcells）限制了免疫病理学，并迅速成为自身免疫和癌症疾病的治疗靶点。使Treg能够在体外诱导、扩增、表征和操作的工具将有助于未来的临床开发。在本章中，我们详细描述了如何使用细菌超抗原从外周血单个核细胞中有效诱导人T细胞活化。还描述了如何评估人Treg表型和抑制能力。提供了技术细节、变化和替代实验条件。
Regulatory T cells (Tregs) that suppress the activation of immune effector cells limit immunopathology and are fast emerging as therapeutic targets for autoimmune and cancer disease. Tools enabling Treg in vitro-induction, expansion, and characterization and manipulation will help future clinical developments. In this chapter, we describe in detail how to use bacterial superantigens to induce human Tregs efficiently from peripheral blood mononuclear cells. How to assess human Treg phenotype and suppressive capacity are also described. Technical details, variations, and alternative experimental conditions are provided.